C4859
Cycloheximide solution
Ready-Made Solution, microbial, 100 mg/mL in DMSO, Suitable for cell culture
동의어(들):
CHX
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제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C15H23NO4
CAS 번호:
Molecular Weight:
281.35
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
12352111
MDL number:
Form:
ready-to-use solution
Quality level:
biological source
microbial
Quality Level
description
suitable for testing specifications (CHO C. growth inhibitor)
form
ready-to-use solution
concentration
100 mg/mL in DMSO
solubility
DMSO: soluble
antibiotic activity spectrum
fungi, yeast
mode of action
protein synthesis | interferes
storage temp.
2-8°C
SMILES string
O=C1[C@@]([C@H](O)CC2CC(NC(C2)=O)=O)([H])C[C@@H](C)C[C@@H]1C
InChI
1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
InChI key
YPHMISFOHDHNIV-FSZOTQKASA-N
Gene Information
human ... FKBP1A(2280), PIN1(5300)
Application
Cycloheximide solution has been used:
- to study its role in modulating cell death induced by tumor necrosis factor α (TNFα) in combination with the second mitochondria-derived activator of caspases (SMAC) mimetic birinapant or interferon-gamma (IFNγ), specifically under conditions that inhibit caspase-8 activity
- in the study to inhibit protein translation during the CHX-chase assays
- to treat antisense oligonucleotide (AON) transfected patient-derived fibroblasts and inhibit the nonsense-mediated decay (NMD) pathway to analyze the therapeutic potential AON and visualize the transcript containing the pseudoexon (PE)
Biochem/physiol Actions
Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors.
Tested for cell growth arrest, selection of cycloheximide resistant yeast, apoptosis induction, and facilitation of apoptosis induction by FasL.
Other Notes
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Hygroscopic. Storage class (TRGS 510): Non-combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials.
signalword
Danger
Hazard Classifications
Acute Tox. 3 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 2 - Repr. 1B - Skin Irrit. 2
저장 등급
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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Matthias Rottmann et al.
Science (New York, N.Y.), 329(5996), 1175-1180 (2010-09-04)
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