C5301
Monoclonal Anti-Cytokeratin Peptide 8 antibody produced in mouse
clone M20, ascites fluid
동의어(들):
Anti-CARD2, Anti-CK-8, Anti-CK8, Anti-CYK8, Anti-K2C8, Anti-K8, Anti-KO
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
M20, monoclonal
Application:
IF, IHC (f), WB
Citations:
24
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
M20, monoclonal
mol wt
antigen 52 kDa
contains
15 mM sodium azide
species reactivity
feline, human, rabbit, canine, bovine
technique(s)
immunohistochemistry (frozen sections): suitable, indirect immunofluorescence: 1:200 using frozen sections of human or animal tissue, western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... KRT8(3856)
General description
Cytokeratin is a 52.5kD cytoplasmic structural protein that belongs to type II, neutral-to-basic subfamily. It consists of 29 different proteins which is the characteristic of epithelial and trichocytic cells. Monoclonal anti-cytokeratin peptide 8 antibody can be used as a marker for epithelial cell differentiation as well as a tool for tumor identification and classification. It can also be used in western blotting. Mouse anti-cytokeratin peptide 8 antibody reacts specifically with various human simple and complex epithelia like- liver, intestine, pancreas, urinary bladder, salivary gland, thyroid, prostate, mesothel, and placenta but not with stratified squamous epithelia. The product also does not react with non-epithelial tissues (exceptions-certain smooth muscle cells). This product has also shown cross reactivity for cytokeratin of rabbit, cow, dog, and cat.Monoclonal Anti-Cytokeratin 8 (mouse IgG1 isotype) is derived from the M20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a cytokeratin preparation purified from the human breast carcinoma cell line MCF7.
Immunogen
cytokeratin from human breast carcinoma cell line MCF7.
Application
Monoclonal anti-cytokeratin peptide 8 antibody has been used in
- immunofluorescence microscopy
- immunohistochemistry
- dot blotting
- protein preparation from bovine epithelial cells
- immunofluorescence
- western blotting
Biochem/physiol Actions
Monoclonal anti-cytokeratin peptide 8 antibody can be used as a marker for epithelial cell differentiation as well as a tool for tumor identification and classification. It can also be used in western blotting.
Mouse anti-cytokeratin peptide 8 antibody reacts specifically with various human simple and complex epithelia like- liver, intestine, pancreas, urinary bladder, salivary gland, thyroid, prostate, mesothel, and placenta but not with stratified squamous epithelia. The product also does not react with non-epithelial tissues (exceptions-certain smooth muscle cells). This product has also shown cross reactivity for cytokeratin of rabbit, cow, dog, and cat.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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저장 등급
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Myoglobin: a promising exogenous reference marker using in proteomics analysis
Wang T, et al.
Food Science and Biotechnology, 22, 393-398 (2013)
K L Simpson et al.
Cell death & disease, 4, e613-e613 (2013-05-04)
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A Bader et al.
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In the vertebrate embryo the heart is the first organ to form. Embryonic and extra-embryonic tissues are supposed to contribute to cardiac lineage commitment before and during gastrulation in a paracrine fashion. Evidence has accumulated that factors secreted by the
Mingxi Hua et al.
Journal of cell science, 125(Pt 23), 5800-5810 (2012-09-15)
The correct functioning of hepatocytes requires the establishment and maintenance of hepatocyte polarity. However, the mechanisms regulating the generation of hepatocyte polarity are not completely understood. The differentiation of human fetal hepatic progenitor cells (hFHPCs) into functional hepatocytes provides a
L A Smith et al.
Frontiers in cell and developmental biology, 9, 667246-667246 (2021-05-25)
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