C7521
Human Collagen Type IV
from human placenta, powder, suitable for substrate for collagenase and for diagnostic assay manufacturing
동의어(들):
Collagen Type 4
제품 이름
Collagen from human placenta, Bornstein and Traub Type IV, powder
biological source
human placenta
description
Sigma Type VI
form
powder
impurities
HIV, hepatitis B and hepatitis C, none detected
solubility
0.5 M acetic acid: 1 mg/mL
suitability
suitable for substrate for collagenase
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Quality Level
Gene Information
human ... COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)
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Biochem/physiol Actions
During development, collagen IV is ubiquitously distributed in BMs. During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.
Preparation Note
This is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C. It is prepared by a modification of the pepsin extraction method of Glanville, R.W., et al.
Analysis Note
An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.
Other Notes
All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains.
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.
저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Handan Xiang et al.
Cell discovery, 3, 17019-17019 (2017-06-20)
Cellular senescence is a cell fate characterized by an irreversible cell cycle arrest, but the molecular mechanism underlying this senescence hallmark remains poorly understood. Through an unbiased search for novel senescence regulators in airway basal cells, we discovered that the
Marion Jeanne et al.
Circulation, 131(18), 1555-1565 (2015-03-11)
Collagen type IV alpha1 (COL4A1) and alpha2 (COL4A2) form heterotrimers critical for vascular basement membrane stability and function. Patients with COL4A1 or COL4A2 mutations suffer from diverse cerebrovascular diseases, including cerebral microbleeds, porencephaly, and fatal intracerebral hemorrhage (ICH). However, the
M L Fulcher et al.
American journal of physiology. Lung cellular and molecular physiology, 296(1), L82-L91 (2008-11-04)
Immortalization of human bronchial epithelial (hBE) cells often entails loss of differentiation. Bmi-1 is a protooncogene that maintains stem cells, and its expression creates cell lines that recapitulate normal cell structure and function. We introduced Bmi-1 and the catalytic subunit
Anuwat Pinyachat et al.
Toxicon : official journal of the International Society on Toxinology, 57(5), 772-780 (2011-02-22)
Molecular cloning and functional characterization of P-III snake venom metalloproteinases (SVMPs) will give us deeper insights in the pathogenesis of viper bites. This may lead to novel therapy for venom-induced local tissue damages, the complication refractory to current antivenom. The
Emma Lång et al.
Nature communications, 9(1), 3665-3665 (2018-09-12)
Epithelial sheet spreading is a fundamental cellular process that must be coordinated with cell division and differentiation to restore tissue integrity. Here we use consecutive serum deprivation and re-stimulation to reconstruct biphasic collective migration and proliferation in cultured sheets of
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