C8487
Anti-Caspase 3, Active antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
동의어(들):
Activated Caspase 3 Antibody, Activated Caspase 3 Antibody - Anti-Caspase 3, Active antibody produced in rabbit, Anti-Apopain, Anti-CPP32, Anti-Yama
제품 이름
Anti-Caspase 3, Active antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rat, bovine, human, pig, canine, mouse
packaging
antibody small pack of 25 μL
technique(s)
indirect immunofluorescence: 1:1,000 using human epitheloid carcinoma HeLa cell line, treated with staurosporine
microarray: suitable
western blot: 1:500 using recombinant human caspase 3, active (Sigma Product No. C1224)
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CASP3(836)
mouse ... Casp3(12367)
rat ... Casp3(25402)
Application
Anti-Caspase 3, Active antibody has been used:
- for western blotting of cytochrome c for caspase activation
- as primary antibody in immunofluorescence staining of embryos and postnatal mice cryosections
- in Western blot analysis of activated caspase 3
- as a primary antibody in immunodetection of rat brain sections
Biochem/physiol Actions
Caspase 3 is one of the key effectors of apoptosis. It acts downstream of caspase 9 in the apoptotic pathway. During apoptosis, it gets activated by proteolytic cleavage into the 17-19 kDa (p17, p18) and 12 kDa (p12) active subunits. Any alteraion in CASP-3 in mice, causes premature lethality. It has also shown that the deletion of gene affects brain development resulting of hyperplasias and disorganized cell deployement. Clinically it is related to Huntington disease (HD). Caspase 3 brings about cell death by activating CAD (caspase activated DNAse I), an endonuclease that causes degradation of chromosomal DNA.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Caspase-3 (CASP3) belongs to the class of cysteine proteases. CASP3 is an executioner caspase, which is cleaved and activated with the help of caspase-8 and caspase-9 initiator caspases. CASP3 is mapped to human chromosome 4q35.1.
Caspases are a family of intracellular proteases that mediate cell death and the principal effectors of apoptosis. There are 14 different caspases have identified which can be grouped into three different subfamilies based on their substrate specificities. Caspase 3 is also known as CPP32, Yama, apopain. Among all the types, caspase 3, a cytosolic protein, found in cells as an inactive 32 kDa proenzyme.
Immunogen
Synthetic peptide corresponding to the cleavage site of human caspase 3 conjugated to KLH.
Physical form
Supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Store at –20 °C. For continuous use, the product may be storage, the product may be stored at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
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저장 등급
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Caspases: an apoptosis mediator
Palai T K and Mishra S R
Journal of Advanced Veterinary and Animal Research, 2(1) (2015)
Expression levels of cleaved caspase-3 and caspase-3 in tumorigenesis and prognosis of oral tongue squamous cell carcinoma
Liu PF, et al.
Testing, 12(7) (2017)
Annaïg Lan et al.
Apoptosis : an international journal on programmed cell death, 12(3), 573-591 (2006-12-30)
The human probiotic Propionibacterium freudenreichii kills colorectal adenocarcinoma cells through apoptosis in vitro via its metabolites, the short chain fatty acids (SCFA), acetate and propionate. However, the precise mechanisms, the kinetics of cellular events and the impact of environmental factors
Paul Delgado-Olguín et al.
Development (Cambridge, England), 141(23), 4610-4617 (2014-11-02)
Maintenance of vascular integrity is required for embryogenesis and organ homeostasis. However, the gene expression programs that stabilize blood vessels are poorly understood. Here, we show that the histone methyltransferase Ezh2 maintains integrity of the developing vasculature by repressing a
Ludger Leifeld et al.
The American journal of pathology, 168(3), 778-785 (2006-03-02)
Apoptosis is a key mechanism underlying fulminant hepatic failure. The role of gelsolin in such apoptotic pathways is not well understood because both pro-apoptotic and anti-apoptotic effects have been reported in vitro, depending on the cell type and in vitro
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