Monoclonal Anti-Neural Cell Adhesion Molecule antibody produced in mouse

Monoclonal Anti-N-CAM (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Neural cell adhesion molecule (N-CAM), the best characterized CAM, exists in adult brain. It belongs to the family of sialoglycoproteins, which arise from alternative splicing of mRNA transcribed from a single gene. Immunocytochemical data indicate that in the adult, N-CAM is expressed mainly in cells of the nervous system. There are indications that N-CAM may also be expressed by non-neural cells in the adult, as it has been found in studies of various embryonic developmental stages.

Mouse monoclonal clone NCAM-0B11 anti-Neural Cell Adhesion Molecule antibody localizes the high molecular weight isoform of N-CAM (neural cell adhesion molecule) in human and several other mammalian species. The antibody shows a strong reaction to N-CAM A/N-CAM 180, however it also recognizes N-CAM B/N-CAM 140. In an immunoblot, the product reacts with polysialated N-CAM in embryonic material. Breakdown products of N-CAM may be stained; they appear as bands lower than 100 kD on the immunoblot.

growth cone enriched plasma membrane fraction from E17 rat forebrain.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (3 papers)

Monoclonal Anti-Neural Cell Adhesion Molecule antibody produced in mouse has been used in:

• Immunofluorescence
• Immunohistochemistry
• Immunoblotting

Neural cell adhesion molecule (N-CAM) are believed to be involved in cell-cell interactions and probably play an important role in embryogenesis and development.

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