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Merck

D1806

Taq DNA Polymerase from Thermus aquaticus

with 10× PCR reaction buffer containing MgCl2

동의어(들):

MgCl2 taq polymerase, Taq polymerase

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
9012-90-2
UNSPSC Code:
12352204
NACRES:
NA.55
EC 번호:
2.7.7.7 (BRENDA, IUBMB)
MDL number:
MFCD00166026

주요 문서

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제품 이름

Taq DNA Polymerase from Thermus aquaticus, with 10× PCR reaction buffer containing MgCl2

biological source

enzyme from bacterial (Thermus Aquaticus)

recombinant

expressed in E. coli

form

liquid

usage

sufficient for 10000 reactions
 sufficient for 3000 reactions
 sufficient for 500 reactions

mol wt

94 kDa

feature

dNTPs included: no
hotstart: no

concentration

5 units/μL

technique(s)

PCR: suitable

color

colorless

input

purified DNA

suitability

suitable for PCR and automated sequencing reactions

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

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관련 카테고리

Application

Taq DNA Polymerase from Thermus aquaticus has been used:
  • in the quantification of fungal growth by polymerase chain reaction (PCR) and photometric assay
  • in conventional reverse transcriptase (RT)-PCR
  • in simple sequence repeats (SSR) genotyping
  • as a component of PCR mix for amplification of genomic and mitochondrial DNA
  • in direct tetra-primer amplification refractory mutation system (T-ARMS) PCR to amplify dried whole blood samples

Biochem/physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

Features and Benefits

  • Low per unit cost of Taq

General description

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. The enzyme is in a recombinant form, expressed in E. coli. It can withstand repeated heating to 95 °C without significant loss of activity. Each lot of Taq DNA Polymerase is tested for PCR amplification and double-stranded sequencing.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Other Notes

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.
Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.

Packaging

Taq DNA Polymerase with 10× reaction buffer containing MgCl2
Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl2 (D1806) or a 10× reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.

저장 등급

12 - Non Combustible Liquids

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시험 성적서(COA)

Lot/Batch Number
0000461601
0000461601
0000461617
0000461617
0000416555

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Fundamental study on the influence of Fusarium infection on quality and ultrastructure of barley malt
Oliveira P M, et al.
International Journal of Food Microbiology, 156(1), 32-43 (2012)
Lactic acid bacteria bioprotection applied to the malting process. Part II: Substrate impact and mycotoxin reduction
Oliveira P, et al.
Food Control, 51, 444-452 (2015)
Human endogenous retrovirus family HERV-K (HML-2) RNA transcripts are selectively packaged into retroviral particles produced by the human germ cell tumor line Tera-1 and originate mainly from a provirus on chromosome 22q11. 21
Ruprecht K, et al.
Journal of Virology, 82(20), 10008-10016 (2008)
Melek Chaouch et al.
Parasite epidemiology and control, 14, e00212-e00212 (2021-05-18)
Leishmaniases are caused by protozoan parasites of the genus Leishmania transmitted by females blood-feeding phlebotomine insects (Diptera: Psychodidae). In Tunisia, cutaneous and visceral leishmaniases are of public health concern. In Tunisia, 17 species of phlebotomine sand flies are described. Here
Severine Hurni et al.
The Plant journal : for cell and molecular biology, 76(6), 957-969 (2013-10-16)
The improvement of wheat through breeding has relied strongly on the use of genetic material from related wild and domesticated grass species. The 1RS chromosome arm from rye was introgressed into wheat and crossed into many wheat lines, as it
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문서

Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

프로토콜

표준 PCR 프로토콜

표준 PCR 프로토콜 단계를 알아보고 시약 목록 또는 사이클링 매개변수를 검토하세요. 일상적인 DNA 증폭을 위한 이 방법은 표준 Taq DNA 중합효소를 사용합니다.

dNTP Hot Start PCR Protocol

Hot Start dNTPs block DNA polymerase until heat activation, enhancing PCR specificity.

Standard PCR Protocol

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

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