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Merck

D4545

Taq DNA Polymerase from Thermus aquaticus

with 10× PCR reaction buffer without MgCl2

동의어(들):

Taq polymerase, Taq polymerase enzyme

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
9012-90-2
UNSPSC Code:
12352204
NACRES:
NA.55
EC 번호:
2.7.7.7 (BRENDA, IUBMB)
MDL number:
MFCD00166026

주요 문서

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제품 이름

Taq DNA Polymerase from Thermus aquaticus, with 10× PCR reaction buffer without MgCl2

biological source

enzyme from bacterial (Thermus Aquaticus)

recombinant

expressed in E. coli

form

liquid

usage

sufficient for 1500 reactions
 sufficient for 250 reactions
 sufficient for 50 reactions
 sufficient for 5000 reactions

feature

dNTPs included: no
hotstart: no

concentration

5 units/μL

technique(s)

PCR: suitable

color

colorless

input

purified DNA

suitability

suitable for PCR and automated sequencing reactions

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

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관련 카테고리

Application

Taq DNA Polymerase from Thermus aquaticus has been used:
  • in the process of DNA extraction (during gene amplification and sequencing)
  • in genotyping
  • in polymerase chain reaction (PCR) to study the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8)
  • for amplification of RNA from primary endothelial cells by conventional PCR

Biochem/physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

Features and Benefits

  • MgCl2 provided in a separate tube to allow MgCl2 optimization
  • Can withstand repeated heating to 95 °C without significant loss of activity

General description

Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus. A stable deoxyribonucleic acid (DNA) polymerase (with a temperature optimum of 80°C) has been purified from the extreme thermophile Thermus aquaticus. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities. Maximal activity of the enzyme needs all four deoxyribonucleotides and activated calf thymus DNA.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Other Notes

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.
Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.

Packaging

Taq DNA Polymerase with 10× reaction buffer without MgCl2. Includes a separate tube of 25 mM MgCl2
Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl2 (D1806) or a 10× reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.

flash_point_f

Not applicable

hcodes

H412

Hazard Classifications

Aquatic Chronic 3

저장 등급

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_c

Not applicable

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시험 성적서(COA)

Lot/Batch Number
0000489786
0000489786
0000463991
0000463991
0000459773

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문서 라이브러리 방문

A Chien et al.
Journal of bacteriology, 127(3), 1550-1557 (1976-09-01)
A stable deoxyribonucleic acid (DNA) polymerase (EC 2.7.7.7) with a temperature optimum of 80 degrees C has been purified from the extreme thermophile Thermus aquaticus. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities. Maximal activity of the
Nick A Bersinger et al.
Fertility and sterility, 89(5 Suppl), 1530-1536 (2007-09-01)
To analyze the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8) by epithelial cells and the response of these cells to cytokine stimulation. In vitro study using eutopic endometrial tissue. University hospital. Cycling women undergoing laparoscopy
Anna Bobrowska et al.
PloS one, 6(6), e20696-e20696 (2011-06-17)
Huntington's disease (HD) is a progressive neurodegenerative disorder for which there is no effective disease modifying treatment. Following-on from studies in HD animal models, histone deacetylase (HDAC) inhibition has emerged as an attractive therapeutic option. In parallel, several reports have
Slobodan Jergic et al.
The EMBO journal, 32(9), 1322-1333 (2013-02-26)
Processive DNA synthesis by the αεθ core of the Escherichia coli Pol III replicase requires it to be bound to the β2 clamp via a site in the α polymerase subunit. How the ε proofreading exonuclease subunit influences DNA synthesis
Ryan E Davey et al.
Stem cells (Dayton, Ohio), 24(11), 2538-2548 (2006-07-11)
Highly ordered aggregates of cells, or niches, regulate stem cell fate. Specific tissue location need not be an obligatory requirement for a stem cell niche, particularly during embryogenesis, where cells exist in a dynamic environment. We investigated autoregulatory fixed-location-independent processes
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문서

Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

중합효소 연쇄 반응

중합효소 연쇄 반응은 분자 생물학에서 가장 널리 사용되는 기술 중 하나로, 변성, 결합, 연장이라는 세 가지 주요 단계를 거칩니다.

프로토콜

표준 PCR 프로토콜

표준 PCR 프로토콜 단계를 알아보고 시약 목록 또는 사이클링 매개변수를 검토하세요. 일상적인 DNA 증폭을 위한 이 방법은 표준 Taq DNA 중합효소를 사용합니다.

Hot Start dNTP Protocol

Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.

dNTP Hot Start PCR Protocol

Hot Start dNTPs block DNA polymerase until heat activation, enhancing PCR specificity.

Standard PCR Protocol

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

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