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Merck

D5030

Dulbecco′s Modified Eagle′s Medium

Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

동의어(들):

DME, DMEM

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제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.75
UNSPSC Code:
12352207
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제품 이름

Dulbecco′s Modified Eagle′s Medium, Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

Application

Dulbecco′s Modified Eagle′s Medium has been used:
  • to culture the isolated flexor digitorum brevis (FDB) fibers to measure exogenous fatty acid (FA) utilization as part of oxygen consumption rate (OCR) measurements
  • to culture the isolated tibialis anterior (TA) muscle fibers for lactate measurements
  • to culture the human bone marrow mesenchymal stem cells for osteogenic differentiation to prepare a low-glucose medium to culture human hepatocellular carcinoma HepG2 cells

General description

Dulbecco′s Modified Eagle′s Medium (DME) is a modification of Basal Medium Eagle (BME) that has been formulated with a 4-fold higher concentration of amino acids and vitamins. It includes additional supplementary components. The DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. Modifying the medium with 4,500 mg/L glucose is optimal for culturing certain cell types.
The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application.

Preparation Note

Formulated to contain 8.3 grams of powder per liter of medium.
Supplement with 1.0 g/L glucose, 0.584 g/L L-glutamine, 3.7 g/L sodium bicarbonate.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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시험 성적서(COA)

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Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.

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