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Merck

D5637

3,3′-Diaminobenzidine tetrahydrochloride hydrate

≥96%

동의어(들):

(1,1′-Biphenyl)-3,3′,4,4′-tetramine tetrahydrochloride dihydrate

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
C12H14N4 · 4HCl · xH2O
CAS 번호:
868272-85-9
NACRES:
NA.47
PubChem Substance ID:
57654109
UNSPSC Code:
12171500
EC Number:
231-018-9
MDL number:
MFCD08273058

주요 문서

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InChI key

DXWSCXIZIHILNP-UHFFFAOYSA-N

InChI

1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2

SMILES string

O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

assay

≥96%

form

powder, crystals or chunks

technique(s)

titration: 96-104% (with NaOH)

color

white to light brown, and Faint Red and Faint Grey to Light Grey

mp

280 °C

solubility

H2O: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

Quality Level

200

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관련 카테고리

General description

3,3′-Diaminobenzidine tetrahydrochloride hydrate is a chromogen commonly used in immunohistochemistry and other staining techniques to visualize the presence of antigens or antibodies in biological samples. DAB HCl is designed as an immunohistology stain to visualize peroxidase activity in cells. DAB oxidation can be measured using a spectrophotometer.

Application

3,3′-Diaminobenzidine tetrahydrochloride hydrate is suitable for immunohistology staining procedures, western blotting, and FISH (fluorescence in situ hybridization). DAB is used in studying epithelial cell apoptosis in the event of early cataracts, and immunohistochemistry protocols of autosomal dominant polycystic kidney disease (PKD).

Biochem/physiol Actions

In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown-colored complex.

signalword

Danger

pictograms

Health hazardExclamation mark
GHS08,GHS07

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

저장 등급

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
MKCT8673
MKCW1123
MKCW2779
MKCV2140
MKCK5099

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문서 라이브러리 방문

Fatima Solange Pasini et al.
Journal of gastroenterology, 49(11), 1453-1466 (2013-11-13)
The TNM Classification of Malignant Tumours (TNM) staging system is the primary means of determining a prognosis for gastric adenocarcinoma (GC). However, tumor behavior in the individual patient is unpredictable and in spite of treatment advances, a classification of 'advanced
Franc Llorens et al.
Nature communications, 11(1), 619-619 (2020-02-01)
The clinical diagnosis of vascular dementia (VaD) is based on imaging criteria, and specific biochemical markers are not available. Here, we investigated the potential of cerebrospinal fluid (CSF) lipocalin 2 (LCN2), a secreted glycoprotein that has been suggested as mediating
Nicole M Derks et al.
Neuroscience research, 66(1), 117-123 (2009-10-17)
Women have higher vulnerability to stress and stress-induced diseases than men. Estrogen may be involved in the control of sex-dependent stress adaptation via estrogen receptors alpha and beta (ERalpha/beta). Urocortin 1 (Ucn1) in the npEW plays an important role in
A new sensitive colorimetric assay for peroxidase using 3,3'-diaminobenzidine as hydrogen donor.
Herzog V and Fahimi HD
Analytical Biochemistry, 55, 554-562 (1973)
Mammalian AMP-activated protein kinase subfamily.
Stapleton D, et al.
The Journal of Biological Chemistry, 271, 611-614 (1996)
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문서

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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