D6883
2′,7′-Dichlorodihydrofluorescein diacetate
≥97% (HPLC), powder, cell-permeable non-fluorescent probe
동의어(들):
2′,7′-Dichlorofluorescin diacetate, DCFH-DA, 2′,7′-Dichlorodihydrofluorescein diacetate
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C24H16Cl2O7
CAS 번호:
Molecular Weight:
487.29
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
12352200
MDL number:
Beilstein/REAXYS Number:
4610273
제품 이름
2′,7′-Dichlorodihydrofluorescein diacetate, ≥97%
InChI
1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
SMILES string
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O
InChI key
PXEZTIWVRVSYOK-UHFFFAOYSA-N
assay
≥97%
form
powder
storage temp.
−20°C
Quality Level
관련 카테고리
General description
2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) is an oxidation-sensitive fluorescent dye used to detect the intracellular reactive oxygen species (ROS) level. DCFH-DA is a cell-permeable non-fluorescent probe, that can be hydrolyzed by intracellular esterase to DCFH, which forms fluorescent DCF when oxidized by the intracellular ROS.
The excitation wavelength is 504 nm and emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however various publications suggest that it is suitable.
Application
2′,7′-Dichlorofluorescin diacetate is suitable for use in:
- sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism
- microplate assay for detecting oxidative products in phagocytic cells
- quantitative multiwell myeloid differentiation assay
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.
Preparation Note
This product should be stored in powdered format at -20°C.
저장 등급
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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문서
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
DISCOVER Bioactive Small Molecules for Nitric Oxide & Cell Stress Research
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