D6944
Anti-Dopamine Transporter (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
동의어(들):
Anti-DAT, Anti-DAT1, Anti-SLC6A3, Anti-Solute carrier family 6, member 3
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunofluorescence
western blot
western blot
Species reactivity:
mouse (predicted), rat, human
Citations:
29
Technique(s):
immunofluorescence: 5-10 μg/mL using SH-SY-5Y cells
western blot: 0.5-1 μg/mL using rat brain tissue extract
western blot: 0.5-1 μg/mL using rat brain tissue extract
Uniprot accession no.:
제품 이름
Anti-Dopamine Transporter (N-terminal) antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~50 kDa
species reactivity
mouse (predicted), rat, human
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.0 mg/mL
technique(s)
immunofluorescence: 5-10 μg/mL using SH-SY-5Y cells
western blot: 0.5-1 μg/mL using rat brain tissue extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SLC6A3(6531)
mouse ... Slc6a3(13162)
rat ... Slc6a3(24898)
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
General description
Solute carrier family 6 member 3 (SLC6A3) is mapped to human chromosome 5p15.33. It is also known as the dopamine transporter (DAT) and has a zinc binding site and 12 transmembrane region.
Dopamine transporter belongs to a family of biogenic amine transporters that include the norepinephrine, serotonin (SERT), and vesicular monoamine transporters. These transporters are members of a larger Na+/Cl--dependent neurotransmitter transporter family.
Immunogen
synthetic peptide corresponding to amino acids 42-59 of rat dopamine transporter conjugated to KLH. This sequence is highly conserved in mouse DAT (single amino acid substitution) and has lower homology (72% identity) to human DAT.
Application
Anti-Dopamine Transporter antibody produced in rabbit is suitable for western blot analysis at a working concentration of 0.5-1μg/mL using rat brain extract (cytosolic S1 fraction) and HEK-293T cells expressing human DAT1. It was used as a primary antibody in western analysis for dopamine transporter in several studies.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Immunocytochemistry (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
DAT regulates dopaminergic-neurotransmission. It is localized on presynaptic nerve terminals and mediates the active reuptake of dopamine from the synapse, thus terminating the neurotransmitter signal. It is the primary site for the action of neurotoxins, such as MPTP, and the drugs of abuse, cocaine and amphetamine. It has been implicated in a number of dopamine-related disorders, including attention deficit hyperactivity disorder (ADHD), bipolar disorder, clinical depression, and alcoholism. DAT may also have a role in selective vulnerability of nigrostriatal dopaminergic neurons in Parkinson′s disease as it can transport dopamine neurotoxins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
저장 등급
10 - Combustible liquids
wgk
WGK 2
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Enrique Garea-Rodríguez et al.
PloS one, 11(2), e0149776-e0149776 (2016-02-24)
Cerebral dopamine neurotrophic factor (CDNF) belongs to a newly discovered family of evolutionarily conserved neurotrophic factors. We demonstrate for the first time a therapeutic effect of CDNF in a unilateral 6-hydroxydopamine (6-OHDA) lesion model of Parkinson's disease in marmoset monkeys.
A Storch et al.
Journal of neural transmission (Vienna, Austria : 1996), 111(10-11), 1267-1286 (2004-10-14)
The carrier molecule that transports dopamine (DA) into dopamine neurons by an electrogenic, Na(+)- and Cl(-)-transport-coupled mechanism is known as the dopamine transporter (DAT). This uptake system is exclusively expressed in DA neurons with significantly higher levels of DAT expression
Kristopher M Kahlig et al.
The Journal of biological chemistry, 279(10), 8966-8975 (2003-12-31)
Dopaminergic neurotransmission is fine-tuned by the rate of removal of dopamine (DA) from the extracellular space via the Na(+)/Cl(-)-dependent DA transporter (DAT). DAT is a target of psychostimulants such as amphetamine (AMPH) and cocaine. Previously, we reported that AMPH redistributes
Symmetrical dimer of the human dopamine transporter revealed by cross-linking Cys-306 at the extracellular end of the sixth transmembrane segment.
Hastrup H, et al.
Proceedings of the National Academy of Sciences of the USA, 98(18), 10055-10060 (2001)
Takayuki Nakagawa et al.
PloS one, 6(9), e24865-e24865 (2011-10-08)
Repeated intermittent exposure to psychostimulants and morphine leads to progressive augmentation of its locomotor activating effects in rodents. Accumulating evidence suggests the critical involvement of the mesocorticolimbic dopaminergic neurons, which project from the ventral tegmental area to the nucleus accumbens
문서
Protein-based drug transporters are expressed in Sf9 cells. Understanding the specific mechanisms of tumor cell transporters is an essential aspect of chemotherapeutic drug design.
관련 콘텐츠
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.