D8130

5,5′-Dithiobis(2-nitrobenzoic acid)

Name: 5,5′-Dithiobis(2-nitrobenzoic acid)
Brand: SIGMA

≥98%, BioReagent, suitable for determination of sulfhydryl groups

동의어(들):

3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellman’s Reagent

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

Linear Formula:

[-SC₆H₃(NO₂)CO₂H]₂

CAS 번호:

69-78-3

Molecular Weight:

396.35

UNSPSC Code:

12352106

NACRES:

NA.26

PubChem Substance ID:

24894189

EC Number:

200-714-4

Beilstein/REAXYS Number:

1896821

MDL number:

MFCD00007140

Assay:

≥98%

Form:

powder

주요 문서

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도움 문의

SMILES string

OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O

InChI key

KIUMMUBSPKGMOY-UHFFFAOYSA-N

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

product line

BioReagent

assay

≥98%

form

powder

mp

240-245 °C (dec.) (lit.)

suitability

suitable for determination of sulfhydryl groups

Quality Level

200

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General description

5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS^-) reacts with Ellman′s reagent (DTNB^2-), to produce 2-nitro-5-thiobenzoate anion (TNB^2-) and one mixed disulfide (R-S-TNB^-). TNB^2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.

Application

Ellman′s reagent maybe used for acetylcholinesterase assay, and to specifically label cysteine residues. It is a sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues.

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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문서 라이브러리 방문

Gene therapy delivering a paraoxonase 1 variant offers long-term prophylactic protection against nerve agents in mice.

Venkaiah Betapudi et al.

Science translational medicine, 12(527) (2020-01-24)

Chemical warfare nerve agents are organophosphorus chemical compounds that induce cholinergic crisis, leaving little or no time for medical intervention to prevent death. The current chemical treatment regimen may prevent death but does not prevent postexposure complications such as brain

Staphylococcal extracellular adherence protein induces platelet activation by stimulation of thiol isomerases.

Anne Bertling et al.

Arteriosclerosis, thrombosis, and vascular biology, 32(8), 1979-1990 (2012-04-28)

Staphylococcus aureus can induce platelet aggregation. The rapidity and degree of this correlates with the severity of disseminated intravascular coagulation, and depends on platelet peptidoglycans. Surface-located thiol isomerases play an important role in platelet activation. The staphylococcal extracellular adherence protein

Quick measurement of protein sulfhydryls with Ellman's reagent and with 4,4'-dithiodipyridine.

Christian K Riener et al.

Analytical and bioanalytical chemistry, 373(4-5), 266-276 (2002-07-12)

Since its introduction in 1959, Ellman's reagent (5,5'-dithio-bis(2-nitrobenzoic acid)) has been the favorite reagent for spectrophotometric measurement of protein sulfhydryls. Meanwhile however, evidence has accumulated that many protein sulfhydryls give an incomplete reaction with Ellman's reagent, even during prolonged assay

Reassessment of Ellman's reagent.

P W Riddles et al.

Methods in enzymology, 91, 49-60 (1983-01-01)

Ellman's reagent: 5,5'-dithiobis(2-nitrobenzoic acid)--a reexamination.

P W Riddles et al.

Analytical biochemistry, 94(1), 75-81 (1979-04-01)

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프로토콜

Enzymatic Assay of Chloramphenicol Acetyltransferase

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

5,5′-Dithiobis(2-nitrobenzoic acid)

≥98%, BioReagent, suitable for determination of sulfhydryl groups

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

SMILES string

InChI key

InChI

product line

assay

form

mp

suitability

Quality Level

설명

General description

Application

안전성 정보

저장 등급

wgk

flash_point_f

flash_point_c

ppe

문서

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