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Merck

E1031

Anti-ERGIC-53/p58 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

동의어(들):

Anti-ERGIC53, Anti-F5F8D, Anti-FMFD1, Anti-LMAN1, Anti-MCFD1, Anti-MR60, Anti-gp58

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
Conjugate:
unconjugated
Clone:
polyclonal
Application:
indirect immunofluorescence
western blot (chemiluminescent)
Species reactivity:
mouse, rat, human
Citations:
58
Technique(s):
indirect immunofluorescence: 5-10 μg/mL using human HepG2 or rat NRK cells
western blot (chemiluminescent): 0.5-1.0 μg/mL using whole cell extract of mouse NIH3T3 cells
Uniprot accession no.:
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제품 이름

Anti-ERGIC-53/p58 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~58 kDa

species reactivity

mouse, rat, human

technique(s)

indirect immunofluorescence: 5-10 μg/mL using human HepG2 or rat NRK cells
western blot (chemiluminescent): 0.5-1.0 μg/mL using whole cell extract of mouse NIH3T3 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

Gene Information

human ... LMAN1(3998)
mouse ... Lman1(70361)
rat ... Lman1(116666)

Application

Anti-ERGIC-53/ (p58) antibody can be used in immunoblotting and immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ERGIC (ER-Golgi intermediate compartment) is related to type I membrane marker protein ERGIC-53 and rat homolog is referred as (p58) . ERGIC is a dynamic membrane system made up of tubulo vesicular clusters near to ER exit site which facilitates transport of protein from ER to Golgi. Anti-ERGIC-53/ (p58) antibody can be used as a primary antibody (diluted 1: 125) in fluorescence microscopy. It may also be used for western blotting. Rabbit anti-ERGIC-53/ (p58) antibodies react specifically with ERGIC-53/ (p58) of rat, mouse and human.

Immunogen

synthetic peptide corresponding to amino acids 158-170 of rat p58 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and human.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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저장 등급

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

AT-1 is the ER membrane acetyl-CoA transporter and is essential for cell viability.
Mary Cabell Jonas, Mariana Pehar
Journal of Cell Science, 23, 3378-3388 (2010)
J Saraste et al.
The Journal of cell biology, 105(5), 2021-2029 (1987-11-01)
A 58-kD cis-Golgi protein has been identified by generating polyclonal antibodies against heavy (cis) Golgi subfractions. Total microsomes isolated from rat pancreatic homogenates were subfractionated to yield a rough microsomal fraction (B1) and three smooth membrane subfractions (B2-B4) enriched in
Adam K Walker et al.
PloS one, 8(11), e81170-e81170 (2013-12-07)
In amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration, TAR DNA binding protein 43 (TDP-43) accumulates in the cytoplasm of affected neurons and glia, where it associates with stress granules (SGs) and forms large inclusions. SGs form in response to
Yu Lan et al.
Development (Cambridge, England), 143(13), 2344-2355 (2016-05-27)
Cleft palate is a common major birth defect for which currently known causes account for less than 30% of pathology in humans. In this study, we carried out mutagenesis screening in mice to identify new regulators of palatogenesis. Through genetic
A Schweizer et al.
The Journal of cell biology, 107(5), 1643-1653 (1988-11-01)
Purified Golgi membranes of the human intestinal adenocarcinoma cell line Caco-2 were used as an antigen to produce a monoclonal antibody, G1/93, which specifically labels a tubulovesicular compartment near the cis side of the Golgi apparatus, including the first cis-cisterna

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