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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
polyclonal
Application:
indirect immunofluorescence
western blot (chemiluminescent)
western blot (chemiluminescent)
Species reactivity:
mouse, rat, human
Citations:
58
Technique(s):
indirect immunofluorescence: 5-10 μg/mL using human HepG2 or rat NRK cells
western blot (chemiluminescent): 0.5-1.0 μg/mL using whole cell extract of mouse NIH3T3 cells
western blot (chemiluminescent): 0.5-1.0 μg/mL using whole cell extract of mouse NIH3T3 cells
Uniprot accession no.:
제품 이름
Anti-ERGIC-53/p58 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~58 kDa
species reactivity
mouse, rat, human
technique(s)
indirect immunofluorescence: 5-10 μg/mL using human HepG2 or rat NRK cells
western blot (chemiluminescent): 0.5-1.0 μg/mL using whole cell extract of mouse NIH3T3 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... LMAN1(3998)
mouse ... Lman1(70361)
rat ... Lman1(116666)
Application
Anti-ERGIC-53/ (p58) antibody can be used in immunoblotting and immunofluorescence.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
ERGIC (ER-Golgi intermediate compartment) is related to type I membrane marker protein ERGIC-53 and rat homolog is referred as (p58) . ERGIC is a dynamic membrane system made up of tubulo vesicular clusters near to ER exit site which facilitates transport of protein from ER to Golgi. Anti-ERGIC-53/ (p58) antibody can be used as a primary antibody (diluted 1: 125) in fluorescence microscopy. It may also be used for western blotting. Rabbit anti-ERGIC-53/ (p58) antibodies react specifically with ERGIC-53/ (p58) of rat, mouse and human.
Immunogen
synthetic peptide corresponding to amino acids 158-170 of rat p58 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and human.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
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저장 등급
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
AT-1 is the ER membrane acetyl-CoA transporter and is essential for cell viability.
Mary Cabell Jonas, Mariana Pehar
Journal of Cell Science, 23, 3378-3388 (2010)
J Saraste et al.
The Journal of cell biology, 105(5), 2021-2029 (1987-11-01)
A 58-kD cis-Golgi protein has been identified by generating polyclonal antibodies against heavy (cis) Golgi subfractions. Total microsomes isolated from rat pancreatic homogenates were subfractionated to yield a rough microsomal fraction (B1) and three smooth membrane subfractions (B2-B4) enriched in
Adam K Walker et al.
PloS one, 8(11), e81170-e81170 (2013-12-07)
In amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration, TAR DNA binding protein 43 (TDP-43) accumulates in the cytoplasm of affected neurons and glia, where it associates with stress granules (SGs) and forms large inclusions. SGs form in response to
Yu Lan et al.
Development (Cambridge, England), 143(13), 2344-2355 (2016-05-27)
Cleft palate is a common major birth defect for which currently known causes account for less than 30% of pathology in humans. In this study, we carried out mutagenesis screening in mice to identify new regulators of palatogenesis. Through genetic
A Schweizer et al.
The Journal of cell biology, 107(5), 1643-1653 (1988-11-01)
Purified Golgi membranes of the human intestinal adenocarcinoma cell line Caco-2 were used as an antigen to produce a monoclonal antibody, G1/93, which specifically labels a tubulovesicular compartment near the cis side of the Golgi apparatus, including the first cis-cisterna
관련 콘텐츠
Datasheet
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| E1031 | 04053252271670 |
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