EMS0001

PNGase Fast

Name: PNGase Fast
Brand: SIGMA

recombinant, expressed in E. coli

동의어(들):

N-Glycosidase F, PNGase F, Peptide N-glycosidase

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:

41131616

Form:

ready-to-use solution

Recombinant:

expressed in E. coli

주요 문서

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도움 문의

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도움 문의

recombinant

expressed in E. coli

conjugate

(N-linked)

grade

Proteomics Grade

form

ready-to-use solution

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

General description

Peptide-N-glycosidase F (PNGase F) belongs to an enzyme family, that are mainly used for the deglycosylation of N-linked glycans.

Application

PNGase Fast may be used to immobilize in order to perform deglycosylation. It may also be used to immobilize onto methacrylate based monolithic support to release the N-linked carbohydrate moieties from glycoproteins.

Biochem/physiol Actions

Peptide-N-glycosidase F (PNGase F) cleaves asparagine-linked high mannose, hybrid and complex oligosaccharides from glycoproteins. It can also deaminate the asparagine to aspartic acid. PNGase Fast enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, to be prepared for downstream chromatography or mass spectrometry analysis. PNGase Fast creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.

저장 등급

10 - Combustible liquids

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

SLBV7894

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

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문서 라이브러리 방문

Multidimensional system enabling deglycosylation of proteins using a capillary reactor with peptide-N-glycosidase F immobilized on a porous polymer monolith and hydrophilic interaction liquid chromatography-mass spectrometry of glycans

Krenkova J, et al.

Journal of Chromatography A, 1216, 3252-3259 (2009)

Oriented immobilization of peptide-N-glycosidase F on a monolithic support for glycosylation analysis.

Jana Krenkova et al.

Journal of chromatography. A, 1322, 54-61 (2013-11-19)

In this paper, we report on a novel oriented peptide-N-glycosidase F (PNGase F) immobilization approach onto methacrylate based monolithic support for rapid, reproducible and efficient release of the N-linked carbohydrate moieties from glycoproteins. The glutathione-S-transferase-fusion PNGase F (PNGase F-GST) was

David A Fischler et al.

Journal of biomolecular techniques : JBT, 30(4), 58-63 (2019-10-11)

There are several methods, both chemical and enzymatic, to release N-linked glycans for structural characterization. One of the most common enzymatic release methods is the use of peptide:N-glycosidase F (PNGase F). A less expensive and quicker alternative has been reported

Jana Krenkova et al.

Journal of chromatography. A, 1216(15), 3252-3259 (2009-03-10)

A reactor with immobilized peptide-N-glycosidase F on a monolithic polymer support in a capillary has been developed that allows fast and efficient release of N-linked glycans from immunoglobulin G molecules. Two different monolithic scaffolds based on poly(glycidyl methacrylate-co-ethylene dimethacrylate) and

N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols

Fischler DA and Orlando R

Journal of biomolecular techniques : JBT, 30, 58-58 (2019)

모든 관련된 서류 보기

문서

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

Correlation of FcR Affinity Chromatography with Glycan Pattern and ADCC Activity of a Therapeutic Antibody

Fast assessment of antibody-dependent cell-mediated cytotoxicity (ADCC) and glycoform pattern of a therapeutic antibody rituximab by FcγRIIIa affinity chromatography.

Released N-Glycan Analysis of a Therapeutic Antibody Using BIOshell™ Glycan Column

This application note describes the released N-Glycan analysis of a monoclonal antibody, cetuximab, labeled with procainamide, using a BIOshell™ Glycan HPLC column.

Quality Attribute Determination of Monoclonal Antibodies

Workflows for monoclonal antibody adalimumab characterization ensure drug safety and efficacy through critical quality attribute analysis.

모두 보기

PNGase Fast

recombinant, expressed in E. coli

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

recombinant

conjugate

grade

form

shipped in

storage temp.

Quality Level

관련 카테고리

설명

General description

Application

Biochem/physiol Actions

안전성 정보

저장 등급

문서

시험 성적서(COA)

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