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Merck

F2518

Human Fibronectin

from human foreskin fibroblasts, powder, suitable for cell culture

동의어(들):

Fibronectin

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352202
NACRES:
NA.75
EC Number:
289-149-2
MDL number:
MFCD00131062
Biological source:
human foreskin fibroblasts
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
Mol wt:
~550 kDa

주요 문서

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제품 이름

Fibronectin human foreskin fibroblasts, lyophilized powder, suitable for cell culture

biological source

human foreskin fibroblasts

product line

BioReagent

form

lyophilized powder

mol wt

~550 kDa

packaging

pkg of 0.5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

impurities

Human Source, none detected (HCV)
 Human Source, none detected (HIV)
 Human Source, none detected (HTLV)
 Human Source, none detected (HbSAg)
 Microbial Contamination, passes test

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

NCBI accession no.

NM_212482.4

UniProt accession no.

P02751

binding specificity

Peptide Source: Collagen

shipped in

ambient

storage temp.

−20°C

Quality Level

200

Gene Information

human ... FN1(2335)

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관련 카테고리

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Application

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells
Fibronectin human foreskin fibroblasts has been used in extracellular matrix binding assay and probiglycan and cleavage assays. It has also been used in ectoderm (neuronal) differentiation and in the preparation of a semi-solid growth medium.

Biochem/physiol Actions

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Fibronectin production by normal human foreskin fibroblasts has been shown to improve two-fold after glucocorticoid treatment.

Disclaimer

Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

Features and Benefits

  • Fibronectin is isolated from human foreskin fibroblasts maintained in serum-free medium.
  • It can be used as a cell attachment factor for various cell types, including epithelial, mesenchymal, neuronal, neural crest, endothelial cells, and fibroblasts.
  • The starting material is tested and found negative for HIV and HBsAg.
  • The product is soluble in sterile water, yielding a clear, colorless solution.
  • It can be stored at -20°C for at least three years.

General description

Fibronectin 1 (FN1) is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Fibronectin is mostly synthesized by fibroblasts and endothelial cells.It is an adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous and form functional units arranged linearly ontwo subunit arms.

Physical form

Lyophilized in CAPS buffered saline

Preparation Note

Store reconstituted solution in working aliquots at -20°C or lower.
dialyzed against chloroform

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
0000420443
0000420443
0000218931
0000218931
0000129869

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Fibronectin binds and enhances the activity of bone morphogenetic protein 1.
Huang G, et al.
The Journal of Biological Chemistry (2009)
Artificial biomaterials for urological tissue engineering
Farhat WA and Geutjes, PJ
Journal of Separation Science (2009)
Fibronectin: a brief overview of its structure, function, and physiology
Proctor RA
Reviews of Infectious Diseases, 317?321-317?321 (1987)
Construction and characterization of a multilayered gingival keratinocyte culture model: the TURK-U model.
Gursoy U K, et al.
Cytotechnology, 68(6), 2345-2354 (2016)
Ramesh B Kasetti et al.
Scientific reports, 7(1), 14951-14951 (2017-11-04)
Increased synthesis and deposition of extracellular matrix (ECM) proteins in the trabecular meshwork (TM) is associated with TM dysfunction and intraocular pressure (IOP) elevation in glaucoma. However, it is not understood how ECM accumulation leads to TM dysfunction and IOP
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문서

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Fibronectin

Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

세포외 기질 단백질 및 세포 배양 최적화를 위한 도구

라미닌, 콜라겐, 피브로넥틴과 같은 세포외 기질 단백질은 세포 배양에서 세포 부착 기질로 사용될 수 있다.

프로토콜

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

관련 콘텐츠

Data Sheet - F2518

Instructions

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