F6140
Anti-Fibronectin Antibody
mouse monoclonal, FN-3E2
동의어(들):
Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF
제품 이름
Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse, clone FN-3E2, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
FN-3E2, monoclonal
mol wt
antigen 240 kDa
contains
15 mM sodium azide
species reactivity
mouse, human, chicken
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect immunofluorescence: 1:400 using cultured chicken fibroblasts
radioimmunoassay: suitable
western blot: suitable
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... FN1(2335)
mouse ... Fn1(14268)
Application
Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse has been used in:
- immunoblot
- immunofluorescent microscopy
- immunocytochemistry
Biochem/physiol Actions
Fibronectin is a soluble plasma protein that regulates tissue repair and thrombus formation. Insoluble fibronectin is expressed in basement membranes and connective tissue matrix and mainly modulates cell adhesion and migration. Alterations in fibronectin have been associated with microvascular thrombosis, disseminated intravascular coagulation, and atherosclerosis .
Fibronectin may enhance cell adhesion and spreading and affect the routes of cell migration both in vivo and in culture. It has been shown that upon malignant transformation many cells lose their surface bound fibronectin.
The antibody labels cellular fibronectin in the pericellular matrix of chick embryo and mouse and human fibroblasts grown in tissue culture. It may be used for immunoaffinity purification of cellular fibronectin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Cellular Fibronectin (mouse IgM isotype) is derived from the hybridoma produced the fusion of mouse myeloma cells and splenocytes from immunized mice. Fibronectin is an extracellular matrix protein composed of two nearly identical disulfide-bound polypeptides with typical molecular weights of 220-240 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and polyclonal antibodies to either form usually cross-react. The protein contains several functionally and structurally distinct domains, which may bind to cell surfaces, collagen, heparin, etc.
Immunogen
fibronectin antigen released in culture by a human breast cancer cell line
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저장 등급
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Fibronectin in cell adhesion and migration via N-glycosylation
Hsiao CT, et al.
Oncotarget, 8(41), 70653-70653 (2017)
Palladin Mediates Stiffness-Induced Fibroblast Activation in the Tumor Microenvironment
McLane JS, et sl.
Biophysical Journal, 109 (2015)
A Vaheri
Schweizerische medizinische Wochenschrift, 110(40), 1437-1440 (1980-10-04)
Fibronectin is a high molecular weight glycoprotein which is found in a soluble form in plasma (cold-insoluble globulin) and other body fluids and in an insoluble form in connective tissues and associated with basement membranes. Soluble fibronectin interacts with denatured
Lisandra E de Castro Brás et al.
Journal of proteomics, 86, 43-52 (2013-05-18)
The extracellular matrix (ECM) is a critical tissue component, providing structural support as well as important regulatory signaling cues to govern cellular growth, metabolism, and differentiation. The study of ECM proteins, however, is hampered by the low solubility of ECM
Thrombospondin-2 facilitates assembly of a type I collagen-rich matrix in marrow stromal cells undergoing osteoblastic differentiation
Alford AI, et al.
Connective tissue research, 54 (2013)
관련 콘텐츠
Datasheet
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