biological source
sugar beets (Beta vulgaris)
type
Type 400-DL
form
lyophilized powder
color
white
solubility
H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow
storage temp.
15-25°C
Quality Level
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Application
Ficoll® 400 is used together with sodium diatrizoate (Paque) to create Ficoll-Paque, a density mixture, used for the separation of cells and organelles, especially blood derived lymphocytes and monocytes. Ficoll 400 may be used for the development of new cell and organelle separation systems. Ficoll 400 is used in tissue engineering and macromolecular conformation research for the development, evaluation and use of macromolecular crowding (MMC) systems and configurations.
Used for cell separation and organelle isolation.
Other Notes
A nonionic synthetic polymer of sucrose.
Dialyzed
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Legal Information
Ficoll is a registered trademark of Cytiva
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Mirna González-González et al.
Biotechnology progress, 30(3), 700-707 (2014-01-23)
Aqueous two-phase systems (ATPS) represent a promising strategy for the recovery of CD133(+) stem cells. This particular type of stem cells has great potential for research and clinical applications. Traditional [polyethylene glycol (PEG), dextran (DEX), and ficoll] and novel (Ucon)
Lucie Kosinova et al.
Islets, 11(6), 129-140 (2019-09-10)
Instant Blood-Mediated Inflammatory Reaction (IBMIR) is a major cause of graft loss during pancreatic islet transplantation, leading to a low efficiency of this treatment method and significantly limiting its broader clinical use. Within the procedure, transplanted islets obstruct intrahepatic portal
Yanyan Xu et al.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 24(4), 406-409 (2010-05-13)
The purity and activity of islets will greatly affect the outcome of xenotransplantation therapy of type 1 diabetes mellitus. To set up an improved method of the isolation and purification of rat islets, which can obtain high-purity, high-yield, and high-viability
Bo Chen et al.
Tissue engineering. Part C, Methods, 19(8), 586-595 (2013-02-21)
Macromolecular crowding (MMC) has been shown to have a beneficial effect on production and maturation of extracellular matrices in a monolayer cell culture model. To explore its potential in tissue engineering, a mixture of Ficoll 70 and Ficoll 400 is
Frank Brand et al.
Acta neuropathologica, 139(1), 175-192 (2019-09-02)
In search of novel genes associated with glioma pathogenesis, we have previously shown frequent deletions of the KIAA1797/FOCAD gene in malignant gliomas, and a tumor suppressor function of the encoded focadhesin impacting proliferation and migration of glioma cells in vitro
프로토콜
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
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