G0282
Granulocyte-Macrophage Colony-Stimulating Factor from mouse
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
동의어(들):
GM-CSF
제품 이름
Granulocyte-Macrophage Colony-Stimulating Factor from mouse, GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biological source
mouse
recombinant
expressed in E. coli
assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
≤0.2 ng/mL EC50 (corresponds to ≥5 × 106 units/mg)
quality
endotoxin tested
mol wt
~15 kDa (125 amino acids including N-terminal methionine)
packaging
pkg of 5 and 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/mg
color
white
solubility
water: soluble, clear, colorless
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
mouse ... CSF2(12981), Csf2(12981)
Analysis Note
The EC50 activity of mouse GM-CSF is tested in culture using murine FDP-1 cells.
Biochem/physiol Actions
Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.
Physical form
Lyophilized from 10 mM acetic acid plus 250 μg BSA.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
M H Heim
Journal of receptor and signal transduction research, 19(1-4), 75-120 (1999-03-11)
The Jak-STAT pathway was originally discovered through the study of interferon induced intracellular signal transduction. Meanwhile, a large number of cytokines, hormones and growth factors have been found to activate Jaks and STATs. Jaks (Janus Kinases) are a unique class
M A Guthridge et al.
Stem cells (Dayton, Ohio), 16(5), 301-313 (1998-10-10)
The process of ligand binding leading to receptor activation is an ordered and sequential one. High-affinity binding of GM-CSF, interleukin 3 (IL-3), and IL-5 to their receptors induces a number of key events at the cell surface and within the
Specificity of signaling by hematopoietic cytokine receptors: instructive versus permissive effects.
R C Skoda
Journal of receptor and signal transduction research, 19(1-4), 741-772 (1999-03-11)
The helical cytokines constitute a family of proteins with a common three-dimensional structure. They exert a wide variety of biological effects with a preference for the hematopoietic system. The effects of helical cytokines are mediated by cell surface receptors, which
A Kelso
Immunology and cell biology, 76(4), 300-317 (1998-09-02)
Cytokines participate in the induction and effector phases of all immune and inflammatory responses. They are therefore obvious tools and targets for strategies designed to promote, inhibit or redirect these responses. However, the complexity of the cytokine network has hindered
Vasiliki Kyrargyri et al.
Glia, 63(4), 549-566 (2014-10-10)
Microglia are CNS resident immune cells and a rich source of neuroactive mediators, but their contribution to physiological brain processes such as synaptic plasticity, learning, and memory is not fully understood. In this study, we used mice with partial depletion
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