G1512
β-Glucuronidase from Helix pomatia
Type H-5, lyophilized powder, ≥400,000 units/g solid
동의어(들):
β-D-Glucuronide glucuronosohydrolase
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
Specific activity:
≥400,000 units/g solid
type
Type H-5
form
lyophilized powder
specific activity
≥400,000 units/g solid
secondary activity
≤40,000 units/g solid sulfatase
storage temp.
−20°C
Quality Level
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General description
β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Application
β-Glucuronidase from Helix pomatia has been used:
- in the enzymatic treatment of urine samples for liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis
- in the quercetin extraction procedure from pig or rat tissues for high performance liquid chromatography (HPLC) analysis
- in the enzymatic deconjugation step in urine and plasma sample preparation for gas chromatography-mass spectrometry analysis (GC-MS)
β-glucuronidase was used to hydrolyze conjugated glucuronide and sulfate metabolites.
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Biochem/physiol Actions
β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Analysis Note
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Other Notes
A further purification of G0876 by gel permeation chromatography.
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
signalword
Danger
저장 등급
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk
WGK 1
Measurement of intact sulfate and glucuronide phytoestrogen conjugates in human urine using isotope dilution liquid chromatography-tandem mass spectrometry with [13C3] isoflavone internal standards
Clarke DB, et al.
Analytical Biochemistry, 309(1), 158-172 (2002)
Denis M Callewaert et al.
Methods in molecular biology (Clifton, N.J.), 610, 435-449 (2009-12-17)
Isoprostanes are a group of prostaglandin-like compounds that are derived in vivo primarily by free radical-mediated peroxidation of arachidonic acid within phospholipids. The resulting isoprostane moieties are rapidly hydrolyzed, metabolized, and excreted. It is now well recognized that isoprostane levels
GC-MS methods for metabolic profiling of microbial fermentation products of dietary polyphenols in human and in vitro intervention studies
Grun CH, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 871(2), 212-219 (2008)
Maaike M Appeldoorn et al.
The Journal of nutrition, 139(8), 1469-1473 (2009-06-06)
Intervention studies with procyanidin (PC)-rich extracts and products such as cocoa and wine suggest protective effects of PC against cardiovascular diseases. However, there is no consensus on the absorption and metabolism of PC dimers. Interestingly, nothing is known about the
Tissue distribution of quercetin in rats and pigs
de Boer VCJ, et al.
The Journal of Nutrition, 135(7), 1718-1725 (2005)
문서
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
프로토콜
Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.
Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.
관련 콘텐츠
Datasheet
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