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Merck

G2267

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle

lyophilized powder, ≥75 units/mg protein

동의어(들):

D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-609-4
MDL number:
EC 번호:
Specific activity:
≥75 units/mg protein
Biological source:
rabbit muscle
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biological source

rabbit muscle

Quality Level

form

lyophilized powder

specific activity

≥75 units/mg protein

composition

Protein, ≥80% biuret

foreign activity

3-Phosphoglyceric phosphokinase ≤0.05%, lactic dehydrogenase, myokinase, and pyruvate kinase ≤0.01%, triosephosphate isomerase ≤0.1%

shipped in

wet ice

storage temp.

−20°C

General description

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein. It is basically called as a glycolytic enzyme.

Application

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:
  • for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
  • to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
  • in anti-aggregation assays
  • in purified GAPDH studies

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway.
Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<18,New>>>.

Physical form

Lyophilized powder containing citrate buffer salts

Other Notes

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.


저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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문서 라이브러리 방문



Oligomerization of a molecular chaperone modulates its activity
Saio T, et al.
eLife, 7(6), e35731-e35731 (2018)
Poly ADP-Ribose Polymerase Inhibition Ameliorates Hind Limb Ischemia Reperfusion Injury in a Murine Model of Type 2 Diabetes
Long C A, et al.
Annals of Surgery, 258(6) (2013)
Role of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in DNA repair
Kosova A A, et al.
The Journal of Biological Chemistry, 82(6), 643-654 (2017)