G5154
Glasgow Minimum Essential Medium
With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
동의어(들):
BHK medium, GMEM, Glasgow′s MEM
제품 이름
Glasgow Minimum Essential Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
L-glutamine: no
phenol red: 0.0213 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)
shipped in
ambient
storage temp.
2-8°C
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General description
For use with adherent kidney cell lines such as baby hamster kidney cells (BHK).
Glasgow Minimum Essential Medium has been developed by modifying the Eagle′s BME medium. This medium can be used to study the genetic factors affecting cell competence.
Application
Glasgow Minimum Essential Medium has been used to culture:
- Hoxb7- green fluorescence protein (GFP) mouse embryonic stem (ES) cell line for the induction of ureteric bud differentiation
- murine E14 Tg2a embryonic stem (mES) cells
- transduced Chinese hamster ovary (CHO) cells for the expression and production of Siglec-1-Fc fusion proteins
Preparation Note
Supplement with 0.292 g/L L-glutamine and Tryptose phosphate broth solution (T 8159) at 100 ml/L of medium.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Marco D'Addio et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(11), e22017-e22017 (2021-10-27)
Cellular interactions between endothelial cells and macrophages regulate macrophage localization and phenotype, but the mechanisms underlying these interactions are poorly understood. Here we explored the role of sialoglycans on lymphatic endothelial cells (LEC) in interactions with macrophage-expressed Siglec-1 (CD169). Lectin-binding
Sacha Robert et al.
International journal of molecular sciences, 22(19) (2021-10-14)
Differentiation of pluripotent stem cells to cardiomyocytes is influenced by culture conditions including the extracellular matrices or similar synthetic scaffolds on which they are grown. However, the molecular mechanisms that link the scaffold with differentiation outcomes are not fully known.
Cinzia Caprio et al.
Disease models & mechanisms (2021-02-21)
The Ezh2 gene encodes a histone methyltransferase of the Polycomb Repressive Complex 2 that methylates histone H3 lysine 27. In this work we asked whether EZH2 has a role in the development of the pharyngeal apparatus and whether it regulates
Taketaro Sadahiro et al.
Cell stem cell, 23(3), 382-395 (2018-08-14)
The mesoderm arises from pluripotent epiblasts and differentiates into multiple lineages; however, the underlying molecular mechanisms are unclear. Tbx6 is enriched in the paraxial mesoderm and is implicated in somite formation, but its function in other mesoderms remains elusive. Here, using
Isabelle Re Nett et al.
EMBO reports, 19(8) (2018-06-14)
Mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) signalling is implicated in initiation of embryonic stem (ES) cell differentiation. The pathway is subject to complex feedback regulation. Here, we examined the ERK-responsive phosphoproteome in ES cells and identified the negative regulator
관련 콘텐츠
Instructions
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