G7765
Gelatin from cold water fish skin
40-50% in H2O
동의어(들):
Gelatine, Teleostean gelatin
biological source
fish skin
form
liquid
mol wt
~60 kDa
contains
0.15% propyl p-hydroxybenzoate as preservative, 0.2% methyl p-hydroxybenzoate as preservative
concentration
40-50% in H2O
technique(s)
ELISA: suitable, cell culture | mammalian: suitable, immunocytochemistry: suitable, western blot: suitable
storage temp.
2-8°C
Quality Level
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General description
Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water.
Gelatin is derived from collagen and is the most abundant protein in the extracellular matrix (ECM) of the tissues. It is a bioactive material. Gelatin consists of matrix metalloproteinase (MMP) sensitive protein sequences.
Application
Gelatin from cold water fish skin has been used in an incubation medium (IM) in immunolabelling of rat brain sections, as a component of rinse buffer in western blotting of various cells, as a blocking agent in immunofluorescent staining of HeLa cells.
Used as a blocking agent in immunochemistry.
Biochem/physiol Actions
Gelatin is cross-linked either chemically or enzymatically to form a three dimensional (3D) hydrogel.
저장 등급
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Nobuo Terada et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 57(9), 883-888 (2009-05-28)
The purpose of this study was to clarify a previously controversial issue concerning glutamate (Glu) immunoreactivity (IR) in the inner segment (IS) of photoreceptors by using in vivo cryotechnique (IVCT) followed by freeze substitution (FS), which enabled us to analyze
Heterogeneous expression of poliovirus receptor-related proteins in human cells and tissues.
Freistadt M S, et al.
Molecular and Cellular Biology, 10(11), 5700-5706 (1990)
Soybean protein concentrate as a protein source for turbot Scophthalmus maximus L
O.J. Day and H.G. Plascencia GonzAlez
Aquaculture (Amsterdam, Netherlands), 4, 221-228 (2000)
Synthesis and characterization of photocrosslinkable hydrogels from bovine skin gelatin
Suvarnapathaki S, et al.
Royal Society of Chemistry Advances, 9(23), 13016-13025 (2019)
OGFOD1, a novel modulator of eukaryotic translation initiation factor 2alpha phosphorylation and the cellular response to stress
Wehner K A, et al.
Molecular and Cellular Biology, 30(8), 2006-2016 (2010)
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