G8132

β-Glucuronidase from limpets (Patella vulgata)

Name: β-Glucuronidase from limpets (<I>Patella vulgata</I>)
Brand: SIGMA

Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid

동의어(들):

β-D-Glucuronide glucuronosohydrolase

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:

9001-45-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-606-8

MDL number:

MFCD00130629

EC 번호:

3.2.1.31 (BRENDA, IUBMB)

Specific activity:

1,000,000-3,000,000 units/g solid

Biological source:

limpet (Patella vulgata)

주요 문서

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도움 문의

biological source

limpet (Patella vulgata)

type

Type L-II

form

lyophilized powder

specific activity

1,000,000-3,000,000 units/g solid

shipped in

wet ice

storage temp.

−20°C

Quality Level

300

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Application

β-Glucuronidase Type L-II has been shown to be a superior enzyme for the hydrolysis of drug-glucuronides from urine.

New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption. Morphine 3-β-D glucuronide was converted to morphine using β-glucuronidase obtained from Patella vulgate.

Analysis Note

Contains sulfatase activity which is inhibited by 0.1 M phosphate.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

저장 등급

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

Comparison of clonazepam compliance by measurement of urinary concentration by immunoassay and LC-MS/MS in pain management population.

Robert West et al.

Pain physician, 13(1), 71-78 (2010-02-02)

Physicians determine patient compliance with their medications by use of urine drug testing. It is known that measurement of benzodiazepines is limited by immunoassay specificity and cutoff limits and therefore does not offer physicians an accurate picture of their patients'

Vectors with the gus reporter gene for identifying and quantitating promoter regions in <I>Saccharomyces cerevisiae</I>

S.V. Marathe & J.E. McEwen

Gene, 154, 105-107 (1994)

Coupling Complete Blood Count and Steroidomics to Track Low Doses Administration of Recombinant Growth Hormone: An Anti-Doping Perspective.

Luca Narduzzi et al.

Frontiers in molecular biosciences, 8, 683675-683675 (2021-06-29)

Growth Hormone (GH) under its human recombinant homologue (rhGH), may be abused by athletes to take advantage of its well-known anabolic and lipolytic properties; hence it is prohibited in sports by the World Anti-Doping Agency. Due to the rapid turnover

Morphine glucuronide hydrolysis: superiority of beta-glucuronidase from Patella vulgata.

J Combie et al.

Clinical chemistry, 28(1), 83-86 (1982-01-01)

beta-Glucuronidase from Patella vulgata, Helix aspersa, Helix pomatia, and bovine liver were evaluated for usefulness in routine hydrolysis of drug-glucuronic acid conjugates from equine urine samples. Factors affecting the reaction rate (enzyme concentration, ligand concentration, temperature, and pH) were optimized.

Catalytic mechanisms of enymatic glycosyl transfer

M.L. Sinnott

Chemical Reviews, 90, 1171-1202 (1990)

모든 관련된 서류 보기

문서

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Analysis of Drugs of Abuse in Urine

Validate urine sample cleanup with HLB SPE for opioid analysis via LC-MS/MS using the Supel^™ Swift HLB 96-well plate.

프로토콜

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

β-Glucuronidase from limpets (Patella vulgata)

Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

biological source

type

form

specific activity

shipped in

storage temp.

Quality Level

관련 카테고리

설명

Application

Biochem/physiol Actions

Analysis Note

Other Notes

안전성 정보

pictograms

signalword

hcodes

pcodes

Hazard Classifications

저장 등급

wgk

flash_point_f

flash_point_c

ppe

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