G8420

β-Glucuronidase from Escherichia coli

Name: β-Glucuronidase from <I>Escherichia coli</I>
Brand: SIGMA

≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder

동의어(들):

β-D-Glucuronide glucuronosohydrolase

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:

9001-45-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-606-8

MDL number:

MFCD00199013

EC 번호:

3.2.1.31 (BRENDA, IUBMB)

Specific activity:

≥20,000 units/mg protein

Recombinant:

expressed in E. coli overproducing strain

주요 문서

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도움 문의

recombinant

expressed in E. coli overproducing strain

form

lyophilized powder

specific activity

≥20,000 units/mg protein

mol wt

290 kDa

composition

, ~25%

storage temp.

−20°C

Quality Level

300

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Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Effective in the hydrolysis of steroid glucuronides.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Analysis Note

Contains buffer salts and stabilizer.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P280 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Lot/Batch Number

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문서 라이브러리 방문

Enzymes

Hans Ulrich Bergmeyer

Methods of Analysis, 2, 460-461 (1974)

Hydrolysis of steroid glucuronides with beta-glucuronidase preparations from bovine liver, Helix pomatia, and E. coli.

V Graef et al.

Clinical chemistry, 23(3), 532-535 (1977-03-01)

We determined the enzymic activity of beta-glucuronidase preparations from bovine liver, Helix pomatia, and Escherischia coli with steroid glucuronides and nonsteroid glucuronides as substrates. We also studied the effect of Na2SO4 on the enzymic hydrolysis of several substrates with the

Heparanase in inflammation and inflammation-associated cancer.

Amichay Meirovitz et al.

The FEBS journal, 280(10), 2307-2319 (2013-02-13)

Recent years have seen a growing body of evidence that enzymatic remodeling of heparan sulfate proteoglycans profoundly affects a variety of physiological and pathological processes, including inflammation, neovascularization, and tumor development. Heparanase is the sole mammalian endoglycosidase that cleaves heparan

Isolation and characterization of an endosperm-specific promoter from wheat (Triticum aestivum L.).

Fei Song et al.

Zeitschrift fur Naturforschung. C, Journal of biosciences, 67(11-12), 611-619 (2013-02-19)

Genes coding for avenin-like proteins (ALP) represent a new family of wheat storage protein genes. To find a wheat endosperm-specific promoter, a 1644-bp fragment upstream of the ALP type-B gene (GenBank accession number JN622144) was isolated. The important promoter elements

Exploring mechanisms of FGF signalling through the lens of structural biology.

Regina Goetz et al.

Nature reviews. Molecular cell biology, 14(3), 166-180 (2013-02-14)

Fibroblast growth factors (FGFs) mediate a broad range of functions in both the developing and adult organism. The accumulated wealth of structural information on the FGF signalling pathway has begun to unveil the underlying molecular mechanisms that modulate this system

모든 관련된 서류 보기

문서

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

프로토콜

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

β-Glucuronidase from Escherichia coli

≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

recombinant

form

specific activity

mol wt

composition

storage temp.

Quality Level

관련 카테고리

설명

Application

Analysis Note

Other Notes

안전성 정보

pictograms

signalword

hcodes

pcodes

Hazard Classifications

저장 등급

wgk

flash_point_f

flash_point_c

ppe

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