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H2126

Hyaluronidase from sheep testes

Name: Hyaluronidase from sheep testes
Brand: SIGMA

Type II, lyophilized powder, ≥300 units/mg solid

동의어(들):

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:

37326-33-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

253-464-3

MDL number:

MFCD00131351

EC 번호:

3.2.1.35 (BRENDA, IUBMB)

Specific activity:

≥300 units/mg solid

기술 서비스

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도움 문의

속성

conjugate

conjugate (Glucosaminoglycan)

Quality Level

200

type

Type II

form

lyophilized powder

specific activity

≥300 units/mg solid

mol wt

55 kDa

storage temp.

−20°C

설명

General description

Hyaluronidase is a hyaluronic acid-metabolizing enzyme. It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.

Application

Hyaluronidase from sheep testes has been used:

in flow cytometry
in the isolation of putative spermatogonial stem cells (SSCs)
to study the effects of magnetic stimulation in magnetic hydrogel properties
in immunohistochemistry to visualize collagen type II distribution
to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings
for the degeneration of hydrogels

Biochem/physiol Actions

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Physical form

Lyophilized powder containing lactose

Other Notes

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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프로토콜

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

문서

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

What is Hyaluronan?

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.