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Merck

H2126

Hyaluronidase from sheep testes

Type II, lyophilized powder, ≥300 units/mg solid

동의어(들):

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
37326-33-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
MFCD00131351
EC 번호:
3.2.1.35 (BRENDA, IUBMB)
Specific activity:
≥300 units/mg solid

주요 문서

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도움 문의

conjugate

conjugate (Glucosaminoglycan)

type

Type II

form

lyophilized powder

specific activity

≥300 units/mg solid

mol wt

55 kDa

storage temp.

−20°C

Quality Level

200

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관련 카테고리

General description

Hyaluronidase is a hyaluronic acid-metabolizing enzyme. It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.

Application

Hyaluronidase from sheep testes has been used:
  • in flow cytometry
  • in the isolation of putative spermatogonial stem cells (SSCs)
  • to study the effects of magnetic stimulation in magnetic hydrogel properties
  • in immunohistochemistry to visualize collagen type II distribution
  • to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings
  • for the degeneration of hydrogels

Biochem/physiol Actions

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Physical form

Lyophilized powder containing lactose

Other Notes

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
0000527794
0000527794
0000497544
0000497544
0000355503

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COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Robert B Struijk et al.
PloS one, 15(3), e0230253-e0230253 (2020-03-17)
Autologous transplantation of spermatogonial stem cells is a promising new avenue to restore fertility in infertile recipients. Expansion of the initial spermatogonial stem cell pool through cell culturing is a necessary step to obtain enough cells for effective repopulation of
Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting
Mouser VHM, et al.
Biofabrication, 8(3), 035003-035003 (2016)
3D bioprinting of methacrylated hyaluronic acid (MeHA) hydrogel with intrinsic osteogenicity
Poldervaart MT, et al.
PLoS ONE, 12(6), e0177628-e0177628 (2017)
The role of the mechanotransduction ion channel candidate Nanchung-Inactive in auditory transduction in an insect ear.
Warren B and Matheson T
The Journal of Neuroscience, 2310-2317 (2018)
Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture
Otto IA, et al.
European Cells & Materials, 35, 132-150 (2018)
모든 관련된 서류 보기

문서

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

What is Hyaluronan?

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

프로토콜

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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