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Merck

H3537

HEPES solution

99.5%, liquid, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

동의어(들):

N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
7365-45-9
UNSPSC Code:
12161700
PubChem Substance ID:
329815199
NACRES:
NA.25
MDL number:
MFCD00006158

주요 문서

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제품 이름

HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

grade

BioPerformance Certified

sterility

0.2 μm filtered

form

liquid

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested, DNase, RNase, Protease, Nickase, free, endotoxin, tested

pH

5.0-6.0

useful pH range

6.8-8.2

cation traces

Fe: <5 ppm, heavy metals (as Pb): <5 ppm

suitability

suitable for molecular biology

application(s)

diagnostic assay manufacturing

Quality Level

200

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관련 카테고리

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

RNAse HEPES has been used:
  • To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets
  • To recover purified ribonucleotide
  • As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
  • As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

저장 등급

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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시험 성적서(COA)

Lot/Batch Number
0000417234
0000417234
0000417239
0000417229
0000417239

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

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