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Merck

H3663

Anti-HA antibody, Mouse monoclonal

clone HA-7, purified from hybridoma cell culture

동의어(들):

Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:
기술 서비스
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제품 이름

Anti-HA antibody, Mouse monoclonal, clone HA-7, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HA-7, monoclonal

concentration

~1 mg/mL

technique(s)

immunocytochemistry: 1.0-2.0 μg/mL
immunoprecipitation (IP): 0.5-2.0 μg
western blot: 0.25-1 μg/mL

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

Quality Level

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Application

Monoclonal Anti-HA antibody produced in mouse has been used:
  • in immunoblotting
  • in immunocytochemistry
  • in the immunoprecipitation

Biochem/physiol Actions

Hemagglutinin (HA) binds to acid binding and is essential for the initiation of virus infection. It is the key target for the generation of vaccine. HA is the dominant antigen against which humoral immune response is provoked. Vaccines development involves the generation of antibody specific to the variable head and the conserved stalk region. Both the anti-stalk and anti-head antibodies may elicit protective function in influenza disease.
The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

General description

Hemagglutinin (HA) is a glycoprotein present in the membrane of influenza virus. HA exists as a trimer and each monomer at its membrane tip has receptor-binding site. Glycosylation event of HA occurs in endoplasmic reticulum. The head region with the receptor-binding site of the HA is variable. The mouse monoclonal Anti-HA, Clone HA-7 recognizes the human influenza virus hemagglutinin (HA), known as the HA tag. The product is reactive with HA-tagged fusion proteins expressed at either the amino or the carboxy terminus of the fusion protein.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide as a preservative.

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저장 등급

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Influenza hemagglutinin and neuraminidase membrane glycoproteins
Gamblin SJ and Skehel JJ
The Journal of Biological Chemistry, 285(37), 28403-28409 (2010)
A newly cloned ClC-3 isoform, ClC-3d, as well as ClC-3a mediates Cd2+-sensitive outwardly rectifying anion currents
Okada T, et al.
Cellular Physiology and Biochemistry, 33(3), 539-556 (2014)
Yumei Zheng et al.
Nature communications, 10(1), 3600-3600 (2019-08-11)
Autophagy depends on the E2 enzyme, Atg3, functioning in a conserved E1-E2-E3 trienzyme cascade that catalyzes lipidation of Atg8-family ubiquitin-like proteins (UBLs). Molecular mechanisms underlying Atg8 lipidation remain poorly understood despite association of Atg3, the E1 Atg7, and the composite
Ribosomal protein L4 interacts with viral protein VP3 and regulates the replication of infectious bursal disease virus
Chen Y, et al.
Virus Research, 211, 73-78 (2016)
Expanded polyglutamine-containing N-terminal huntingtin fragments are entirely degraded by mammalian proteasomes
Juenemann K, et al.
The Journal of Biological Chemistry, 288(38), 27068-27084 (2013)

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