H4777
Heparan sulfate proteoglycan
≥400 μg/mL glycosaminoglycan
동의어(들):
HSPG
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Biological source:
mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)
Form:
solution
Technique(s):
cell culture | mammalian: suitable
Concentration:
≥400 μg/mL glycosaminoglycan
Mol wt:
≥400 kDa
biological source
mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)
description
For cell culture use., Isolated from basement membrane of Engelbreth-Holm-Swarm mouse sarcoma., Solution in 50 mM Tris HCl, 150 mM NaCl, 1 mM EDTA, 0.1 mM PMSF, pH 7.4, containing ≥400 μg protein per ml.
form
solution
mol wt
≥400 kDa
packaging
pkg of 0.1 mg
concentration
≥400 μg/mL glycosaminoglycan
technique(s)
cell culture | mammalian: suitable
impurities
≥100 μg/mL Uronic acid
NCBI accession no.
UniProt accession no.
binding specificity
Peptide Source: Collagen Type IV
, Peptide Source: Fibronectin
, Peptide Source: Laminin
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
mouse ... Hspg2(15530)
General description
Heparan sulfate proteoglycan (HSPG), a glycoprotein, is an integral part of the basement membrane. It is composed of a core protein covalently bound to heparan sulfate chains. There are three groups of HSPGs: membrane HSPGs, secreted extracellular matrix HSPGs, and the secretory vesicle proteoglycan.
Application
Heparan sulfate proteoglycan has been used:
- in phosphate buffered saline (PBS) and added to fresh citrated blood samples to achieve trauma-equivalent concentrations in a research study
- solid phase binding assay
- for fabrication of extracellular matrix (ECM) microarray slides
Biochem/physiol Actions
Heparan sulfate proteoglycans (HSPGs) have multiple functions in various biological processes. They contribute to basement membrane structure and provide a matrix for cell migration. HSPGs in secretory vesicles, such as serglycin, play a role in packaging granular contents, maintaining proteases activity, and regulating biological activities after secretion. HSPGs can bind and protect cytokines, growth factors, and chemokines, facilitating the formation of gradients essential for cell specification and leukocyte recruitment. They act as receptors for proteases and protease inhibitors, regulate cell-ECM attachment, cell-cell interactions, and cell motility, and can cooperate with growth factor receptors. HSPGs also function as endocytic receptors for ligand clearance and are involved in lipoprotein metabolism and morphogen gradient formation. It also acts as a docking molecule for matrilysin and other matrix metalloproteinases. Heparan Sulfate promotes the attachment of cells in culture and induces high-affinity binding of FGF-basic to cells deficient in heparin sulfate and soluble FGF receptors at a concentration of 10-100 ng/mL.
Preparation Note
This product is isolated from the basement membrane of Engelbreth-Holm-Swarm mouse sarcoma in a solution containing 50 mM Tris HCl at pH 7.4, 150 mM NaCl, 1 mM EDTA, and 0.1 mM PMSF.
Other Notes
Heparan sulfate proteoglycan is a large biomolecule composed of a core protein covalently bound to heparin sulfate chains that is an integral part of the basement membrane. The number of polysaccharide chains and the size of the core protein are variable according to the source.
Disclaimer
It is recommended to store this product at 20°C, where it will retain its function for 3 years
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
C Battaglia et al.
European journal of biochemistry, 208(2), 359-366 (1992-09-01)
A large, low-density form of heparan sulfate proteoglycan was isolated from the Engelbreth-Holm-Swarm (EHS) tumor and demonstrated to bind in immobilized-ligand assays to laminin fragment E3, collagen type IV, fibronectin and nidogen. The first three ligands mainly recognize the heparan
Pongsak Khunrae et al.
Journal of molecular biology, 397(3), 826-834 (2010-01-30)
Plasmodium falciparum malaria remains one of the world's leading causes of human suffering and poverty. Each year, the disease takes 1-3 million lives, mainly in sub-Saharan Africa. The adhesion of infected erythrocytes (IEs) to vascular endothelium or placenta is the
Glycocalyx components affect platelet function, whole blood coagulation, and fibrinolysis: an in vitro study suggesting a link to trauma-induced coagulopathy
Martin W Britten, et al.
BMC anesthesiology, 21(1), 83-83 (2021)
Heparan sulfate proteoglycans
Stephane Sarrazin , William C Lamanna, Jeffrey D Esko
Cold Spring Harbor Perspectives in Biology, 3(7) (2011)
Thomas M Clausen et al.
The Journal of biological chemistry, 287(28), 23332-23345 (2012-05-10)
Malaria is a major global health problem. Pregnant women are susceptible to infection regardless of previously acquired immunity. Placental malaria is caused by parasites capable of sequestering in the placenta. This is mediated by VAR2CSA, a parasite antigen that interacts
문서
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
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