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Merck

H7523

HEPES

BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

동의어(들):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C8H18N2O4S
CAS 번호:
7365-45-9
Molecular Weight:
238.30
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24895796
EC Number:
230-907-9
Beilstein/REAXYS Number:
883043
MDL number:
MFCD00006158

주요 문서

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InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

product line

BioXtra

assay

≥99.5% (titration)

form

powder

impurities

Insoluble matter, passes filter test

ign. residue (900°C)

≤0.1% (as SO4)

loss

≤1.0% loss on drying, 110°C

pH

5.0-6.5 (1 M in H2O)

Quality Level

400

solubility

H2O: 1 M, clear, colorless

anion traces

chloride (Cl-): ≤0.05%

cation traces

Al: ≤0.0005%, As: ≤0.0001%, Ba: ≤0.0005%, Bi: ≤0.0005%, Ca: ≤0.001%, Cd: ≤0.0005%, Co: ≤0.0005%, Cr: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Li: ≤0.0005%, Mg: ≤0.0005%, Mn: ≤0.0005%, Mo: ≤0.0005%, Na: ≤0.005%, Ni: ≤0.0005%, Pb: ≤0.0005%, Sr: ≤0.0005%, Zn: ≤0.0005%

absorption

≤0.035 at 280 in H2O at 1 M, ≤0.045 at 260 in H2O at 1 M

application(s)

diagnostic assay manufacturing

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관련 카테고리

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
  • As a component of homogenisation buffer used in protein sample preparation
  • To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation
  • As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation
  • As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

저장 등급

11 - Combustible Solids

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000522058
0000522058
0000506630
0000506630
0000511097

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

The inhibitory effect of intracerebroventricularly injected interleukin 1? on testosterone secretion in the rat: role of steroidogenic acute regulatory protein.
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Biology of Reproduction, 60(2), 527-533 (1999)
In vitro tubulogenesis of endothelial cells by relaxation of the coupling extracellular matrix-cytoskeleton.
Deroanne CF, et al.
Cardiovascular Research, 49(3), 647-658 (2001)
Intervertebral disc regeneration in an ex vivo culture system using mesenchymal stem cells and platelet-rich plasma.
Chen WH, et al.
Biomaterials, 30(29), 5523-5533 (2009)
Hsp60-induced tolerance in the rotifer Brachionus plicatilis exposed to multiple environmental contaminants.
Wheelock CE, et al.
Archives of Environmental Contamination and Toxicology, 36(3), 281-287 (1999)
Increased expression of reticulon 3 in neurons leads to reduced axonal transport of $\beta$ site amyloid precursor protein-cleaving enzyme 1.
Deng M, et al.
The Journal of Biological Chemistry, 288(42), 30236-30245 (2013)
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관련 콘텐츠

HEPES Specification Comparison

HEPES Specification Comparison

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