H9658
Monoclonal Anti-HA antibody produced in mouse
clone HA-7, ascites fluid
동의어(들):
Monoclonal Anti-HA, HA Antibody - Monoclonal Anti-HA antibody produced in mouse, Ha Antibody Sigma, Sigma Ha Antibody, Anti-HA, Anti-Influenza Hemagglutinin
제품 이름
Monoclonal Anti-HA antibody produced in mouse, clone HA-7, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
HA-7, monoclonal
contains
15 mM sodium azide
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Application
Monoclonal Anti-HA antibody produced in mouse has been used in 293T cells for:
- immunoprecipitation in human embryonic kidney 293 cells
- immunoblotting in HeLa cells
- immunopurification in human embryonic kidney 293 cells
- immunostaining for electron microscopy in mouse embryonic fibroblasts
- in immunocytochemistry of hippocampal slices
Monoclonal Anti-HA antibody produced in mouse has been used in:
- Immunoblotting, to study the expression of fusion protein
- Coimmunoprecipitation assay
- Immunoblotting of lysate protein
Biochem/physiol Actions
Hemagglutinin (HA) is the surface glycoprotein of influenza virus. HA is crucial for virus entry and interaction with N-acetylneuraminic acid (Neu5Ac) in host cell surface. HA coordinates with neuraminidase (NA) and facilitates viral entry into host. HA exists as a trimer and each monomer has stalk transmembrane and globular regions. The globular region contains carbohydrate side chains crucial for infecting host.
Influenza virus hemagglutinin (HA) acts as a key immunogen in avian influenza virus (AIV) vaccines. Activated HA facilitates virus attachment to oligosaccharide receptors on host cell surface and is involved in penetration through fusion following conformational modifications in the endolysosomal compartment. 226 and 228 residues in hemagglutinin (HA) of the H3 subtype play a key role in host range restriction and receptor specificity. Influenza virus hemagglutinin (HA) is responsible for 1918 influenza pandemic.
The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
The gene encoding influenza virus hemagglutinin (HA) precursor is mapped on segment 4. It encodes a 76kDa surface glycoprotein which exists as trimers on the viral envelope.
Immunogen
synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH
Preparation Note
For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.
For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.
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저장 등급
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Calmodulin shuttling mediates cytonuclear signaling to trigger experience-dependent transcription and memory
Cohen SM, et al.
Nature Communications, 9(1), 2451-2451 (2018)
Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls.
Fouchier R A M, et al.
Journal of Virology, 79(5), 2814-2822 (2005)
Synaptic targeting and functional modulation of GluK1 kainate receptors by the auxiliary neuropilin and tolloid-like (NETO) proteins.
Copits B A, et al.
The Journal of Neuroscience, 31(20), 7334-7340 (2011)
Visualization of Alternative Functional Configurations of Influenza Virus Hemagglutinin Facilitates Rapid Selection of Complementing Vaccines in Emergency Situations.
Metwally A and Yousif A
International Journal of Molecular Sciences, 18(4), 766-766 (2017)
Sumoylation of histone deacetylase 1 regulates MyoD signaling during myogenesis
Joung H, et al.
Experimental & Molecular Medicine, 50(1), e427-e427 (2018)
관련 콘텐츠
Instructions
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