IgG from human serum

Purified human IgG has been used :-

• as blocking antibody for flow cytometry
• as negative control in chromatin immunoprecipitation assays
• to precipitate IgG in human larynx epidermoid carcinoma cells by immunoprecipitation
• as a standard in enzyme-linked immunosorbent assay

Immunoglobulin G (IgG) regulates the immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG1 regulates complement fixation in mice.

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IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Human IgG is purified from normal human serum by precipitation and gel filtration techniques.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide