I3146
ITS Liquid Media Supplement (100×)
liquid, sterile-filtered, BioReagent, suitable for cell culture
동의어(들):
Human ITS Liquid Media, Insulin-transferrin-sodium selenite
sterility
sterile-filtered
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
impurities
endotoxin, tested
shipped in
ambient
storage temp.
2-8°C
Quality Level
Application
ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:
- α mouse liver 12 (AML-12) cells
- rat fetal testis explants
- human dental pulp stem cells (DPSC)
Biochem/physiol Actions
- Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.
- Transferrin: an iron-transport protein.
- Selenium: an essential trace element normally provided by serum.
Preparation Note
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
Other Notes
Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Leonardo Ricotti et al.
Biomedical materials (Bristol, England), 6(3), 031001-031001 (2011-05-14)
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Kelvin S C Cheung et al.
PloS one, 9(6), e98063-e98063 (2014-06-04)
MicroRNAs (miRs) play a pivotal role in a variety of biological processes including stem cell differentiation and function. Human foetal femur derived skeletal stem cells (SSCs) display enhanced proliferation and multipotential capacity indicating excellent potential as candidates for tissue engineering
Cyrill Géraud et al.
Hepatology (Baltimore, Md.), 52(1), 313-326 (2010-06-26)
Liver sinusoidal endothelium (LSEC) is a prime example of organ-specific microvascular differentiation and functions. Disease-associated capillarization of LSEC in vivo and dedifferentiation of LSEC in vitro indicate the importance of the hepatic microenvironment. To identify the LSEC-specific molecular differentiation program
Scaffold-free cartilage tissue engineering with a small population of human nasoseptal chondrocytes.
Loraine L Y Chiu et al.
The Laryngoscope, 127(3), E91-E99 (2016-11-20)
Cartilage tissue engineering is a promising approach to provide suitable materials for nasal reconstruction; however, it typically requires large numbers of cells. We have previously shown that a small number of chondrocytes cultivated within a continuous flow bioreactor can elicit
문서
Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.
Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.
관련 콘텐츠
Instructions
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