L2164
Anti-Luciferase antibody, Mouse monoclonal
clone LUC-1, purified from hybridoma cell culture
동의어(들):
Anti-Luciferase Antibody
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
LUC-1, monoclonal
Application:
ICC, WB
Citations:
31
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
LUC-1, monoclonal
form
buffered aqueous solution
mol wt
60 kDa
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~2 mg/mL
technique(s)
immunocytochemistry: 20-40 μg/mL using transfected 293Tcells expressing luciferase, immunocytochemistry: suitable, western blot: 2-4 μg/mL using whole extract of transfected 293T cells expressing luciferase, western blot: suitable
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
Anti-Luciferase antibody, Mouse monoclonal , (mouse IgG1 isotype) is derived from the LUC-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with firefly (Photinus pyralis) luciferase. Luciferase, a reporter protein contains two binding pockets, one pocket for ATP and other one for luciferin substrate. Both the pockets are in close proximity to each other.
The mouse monoclonal Anti-Luciferase antibody recognizes the recombinant luciferase in transfected cells. This antibody provides user with an alternative detection method for luciferase. Since it directly detects the luciferase protein, it is not dependent on either the luciferase activity or the measeurement of rapid reaction kinetics. This also allows for the detection of the luciferase enzyme expression in situ with reproducibility between sample sets.
Application
Anti-Luciferase antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunocytochemistry
- immunohistochemistry
Biochem/physiol Actions
Anti-Luciferase antibody, Mouse monoclonal recognizes recombinant luciferase in transfected eukaryotic (293T) cells.
Luciferase catalyzes a bioluminescent reaction in the presence of substrate luciferin as well as Mg2+, oxygen and ATP. The luciferase assay is rapid, sensitive, and unlike the CAT assay, does not require a radioactive substrate. Anti-Luciferase antibody can detect the luciferase enzyme expression in situ, providing a means to study the cellular localization of the signal sequence activation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in a frost-free freezer, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Working Dilution: 1-2 μg/ml
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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저장 등급
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Dun Jack Fu et al.
Translational vision science & technology, 9(13), 44-44 (2021-01-15)
The purpose of this study was to develop and characterize a novel bioluminescence transgenic mouse model that facilitates rapid evaluation of genetic medicine delivery methods for inherited and acquired corneal diseases. Corneal expression of the firefly luciferase transgene (luc2) was
The Lac repressor provides a reversible gene expression system in undifferentiated and differentiated embryonic stem cell
Caron L, et al.
Cellular and Molecular Life Sciences, 62(14), 1605-1612 (2005)
Sung-Yong Kim et al.
Plant physiology, 142(3), 984-992 (2006-09-12)
Extracellular ATP (eATP) in animals is well documented and known to play an important role in cellular signaling (e.g. at the nerve synapse). The existence of eATP has been postulated in plants; however, there is no definitive experimental evidence for
In vivo natriuretic peptide reporter assay identifies chemical modifiers of hypertrophic cardiomyopathy signalling
Becker JR, et al.
Cardiovascular Research, 93(3), 463-470 (2012)
Jie Gao et al.
Plant signaling & behavior, 16(7), 1916211-1916211 (2021-05-27)
Aluminum (Al) toxicity in acidic soils severely reduces crop production worldwide. Sorghum (Sorghum bicolor L.) is an important agricultural crop widely grown in tropical and subtropical regions, where Al toxicity is prevalent. ATP-binding cassette (ABC) transporters play key roles in
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