L7395
Lipoxidase from Glycine max (soybean)
Type I-B, lyophilized powder, ≥50,000 units/mg solid
동의어(들):
15-lipoxygenase, LOX A, Linoleate:oxygen oxidoreductase, Lipoxygenase
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-853-1
MDL number:
EC 번호:
Specific activity:
≥50,000 units/mg solid
General description
Lipoxidases (LOXs), or lipoxygenases, are nonheme iron proteins. Lipoxygenases are widely present in plants, some microorganisms, and animals. They are categorized as LOX-1, LOX-2, and LOX-3 with respect to pH and optimum temperature, substrate specificity, and level of inhibition by different agents.
Application
Lipoxidase from Glycine max (soybean) has been used:
- to study the effects of 2-(α-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol(TMG) on oxidation of lipoproteins
- as a representative enzyme for lipoxygenases in microplate assay platform to screen for enzymes degrading wood extractives
- as a positive control in lipoxygenase inhibitory activity assay
The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).
Biochem/physiol Actions
Lipoxidases are deactivated by heat treatment near 100°C for 5 to 10 minutes. Studies show that gamma irradiation treatment decreases the specific activity of soybean lipoxygenases. These enzymes are used in studies related to asthma and allergies. Lipoxidases are applicable for industrial bio-transformations such as in the production of aroma fine chemicals.
Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.
Physical form
Contains stabilizer and NaCl
Other Notes
One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Norimasa Yoshida et al.
Atherosclerosis, 162(1), 111-117 (2002-04-12)
A novel vitamin E derivative that is freely soluble in water, 2-(alpha-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol (TMG), was evaluated for ability to inhibit development of atherosclerosis in Watanabe heritable hyperlipidemic (WHHL) rabbits or cholesterol-loaded New Zealand White rabbits. Although TMG rapidly entered the circulation
Sereyvath Yoeun et al.
Frontiers in chemistry, 6, 500-500 (2018-11-15)
Allene oxide cyclase (AOC) is a key enzyme in the jasmonic acid (JA) biosynthetic pathway in plants, during which it catalyzes stereospecific conversion of 12,13(S)-epoxy-9(Z),11,15(Z)-octadecatrienoic acid (12,13-EOT) to cis(+)-12-oxophytodienoic acid. Here, rice allene oxide cyclase (OsAOC) was localized to the
Stabilization of Lipoxygenase-1 from Glycine max by Microencapsulation
Lakshmi MC, et al.
Drying Technology, 33(4), 493-501 (2015)
Chemical composition and lipoxygenase activity in soybeans (Glycine max L. Merr.) submitted to gamma irradiation
de Barros EA, et al.
Radiation Physics and Chemistry, 98, 29-32 (2014)
Microplate enzyme assay for screening lipoxygenases to degrade wood extractives
Nguyen D, et al.
Biocatalysis and Biotransformation, 25(2-4), 202-210 (2007)
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