L8754
Lectin from Phaseolus vulgaris (red kidney bean)
Phytohemagglutinin PHA-P, lyophilized powder
동의어(들):
Phaseolus vulgaris agglutinin, PHA
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352202
EC Number:
232-718-7
NACRES:
NA.32
Form:
lyophilized powder
Biological source:
Phaseolus vulgaris
biological source
Phaseolus vulgaris
Quality Level
form
lyophilized powder
potency
≤62.5 μg per mL agglutination activity, ≤10 μg/mL mitogenic activity
impurities
salt, free
storage temp.
2-8°C
General description
PHAs are carbohydrate-binding proteins. There are several types of PHAs identified, predominantly in leguminous plants.
Phytohaemagglutinin (PHA, or phytohemagglutinin) is a lectin found in plants (esp. in legumes). It is obtained from Phaseolus vulgaris (red kidney beans) and acts as a mitogen in human leucocyte culture.
Application
It has also been used as an antigen to measure in vivo T-cell-mediated immune responsiveness.
Lectin from Phaseolus vulgaris (red kidney bean) has been used in phytohemagglutinin (PHA) assay and during assessment of cellular and humoral immunity.
Biochem/physiol Actions
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
Analysis Note
Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
문서
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
Andrew Z Wang et al.
The Journal of experimental biology, 223(Pt 20) (2020-09-13)
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Qianting Yang et al.
Thorax, 75(7), 576-583 (2020-03-24)
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A R S Badari Nath et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 134, 143-147 (2014-07-10)
Phytohaemagglutinin (PHA) is a lectin obtained from Phaseolus vulgaris (red kidney beans), that acts as a mitogen in human leucocyte culture and is commercially available from Gibco. This PHA (Gibco) was found to be very expensive, hence other inexpensive sources