M0275
Minimum Essential Medium Eagle
With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x
동의어(들):
EMEM, MEM
제품 이름
Minimum Essential Medium Eagle, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
Quality Level
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Application
Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera. The L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.
Minimum Essential Medium Eagle has been used for the formulation of collagen methacrylamide (hydrogel). It has also been used to maintain articular cartilage of bovine knee joints, which was later used for MRI (magnetic resonance imaging) study and histological analysis.
Preparation Note
Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Diffusion tensor imaging of articular cartilage as a measure of tissue microstructure
Meder R, et al.
Osteoarthritis and Cartilage, 14(9), 875-881 (2006)
A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine
Drzewiecki KE, et al.
Technology, 5(04), 185-195 (2017)
Comparison of morphology, orientation, and migration of tendon derived fibroblasts and bone marrow stromal cells on electrochemically aligned collagen constructs
Gurkan UA, et al.
Journal of Biomedical Materials Research Part A, 94(4), 1070-1079 (2010)
Karl Norris et al.
Micromachines, 10(1) (2019-01-24)
Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial
Collagen-lactoferrin fibrillar coatings enhance osteoblast proliferation and differentiation
Vandrovcova M, et al.
Journal of Biomedical Materials Research Part A, 103(2), 525-533 (2015)
문서
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
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