M2320
Anti-MAP2 Antibody
mouse monoclonal, AP-20
동의어(들):
Anti-MAP2 (2a+2b) antibody, Mouse monoclonal, Anti-Microtubule Associated Protein 2
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
AP-20, monoclonal
Application:
immunocytochemistry
microarray
western blot
microarray
western blot
Species reactivity:
Xenopus, mouse, quail, human, bovine, rat, aquatic salamander
Citations:
44
Technique(s):
immunocytochemistry: suitable
microarray: suitable
western blot: 1-3 μg/mL using rat brain preparation or rat cerebral cortex extract
microarray: suitable
western blot: 1-3 μg/mL using rat brain preparation or rat cerebral cortex extract
Uniprot accession no.:
제품 이름
Anti-MAP2 (2a+2b) antibody, Mouse monoclonal, ~2 mg/mL, clone AP-20, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
AP-20, monoclonal
form
buffered aqueous solution
mol wt
apparent mol wt 280 kDa
species reactivity
Xenopus, mouse, quail, human, bovine, rat, aquatic salamander
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable
microarray: suitable
western blot: 1-3 μg/mL using rat brain preparation or rat cerebral cortex extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MAP2(4133)
mouse ... Mtap2(17756)
rat ... Map2(25595)
유사한 제품을 찾으십니까? 방문 제품 비교 안내
Application
Monoclonal Anti-MAP2 (2a+2b) antibody produced in mouse has been used in immunocytochemistry.
Mouse monoclonal clone AP-20 anti-MAP2 (2a+2b) antibody is used to tag MAP2 for detection and quantitation by Western blotting and immunohistochemical (IHC) techniques. It is used as a probe to determine the roles of MAP2a and MAP2b in cell signaling especially in neural tissues.
Biochem/physiol Actions
Microtubule associated protein 2 (MAP2) is known to promote microtubule assembly and to form side arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton. MAP2 is degraded by a cathepsin D like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in others. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton.
Monoclonal Anti-MAP2 antibody reacts with human, bovine, rat, mouse, Xenopus, salamander, and quail tissue or cells by immunocytochemical techniques utilizing either a fluorescent or peroxidase label, or by immunohistochemistry.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20 MAP2a appears, at levels equal to that of MAP2b. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in others.
Monoclonal Anti-MAP2 (2a + 2b) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with bovine microtubule associated protein 2 (MAP2).
Monoclonal Anti-MAP2 specificity was established by an immunoblot. The antibody localizes the high molecular weight forms of MAP2, namely MAP2a and MAP2b, but shows no reactivity with MAP2c. No crossreactivity is observed with MAP1, MAP5, tubulin, or tau (t).
Immunogen
bovine MAP2
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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저장 등급
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Shinya Hidano et al.
mBio, 7(6) (2016-11-12)
The local production of gamma interferon (IFN-γ) is important to control Toxoplasma gondii in the brain, but the basis for these protective effects is not fully understood. The studies presented here reveal that the ability of IFN-γ to inhibit parasite
The projection domain of MAP2b regulates microtubule protrusion and process formation in Sf9 cells
Belanger D, et al.
Journal of Cell Science, 115(7), 1523-1539 (2002)
Neurons Derived from Human Induced Pluripotent Stem Cells Integrate into Rat Brain Circuits and Maintain Both Excitatory and Inhibitory Synaptic Activities
Yin X, et al.
eNeuro, 6(4) (2019)
Antidepressant drugs diversely affect autophagy pathways in astrocytes and neurons?dissociation from cholesterol homeostasis
Zschocke J, et al.
Neuropsychopharmacology, 36(8), 1754-1754 (2011)
J Lavaur et al.
Cell death discovery, 2, 16018-16018 (2016-08-24)
Noble gases such as xenon and argon have been reported to provide neuroprotection against acute brain ischemic/anoxic injuries. Herein, we wished to evaluate the protective potential of these two gases under conditions relevant to the pathogenesis of chronic neurodegenerative disorders.
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