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Merck

M3024

Minimum Essential Medium Eagle

with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture

동의어(들):

EMEM, MEM

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제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.75
UNSPSC Code:
12352207
기술 서비스
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Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

Earle’s salts (5% CO2): yes
L-glutamine: no
HEPES: no
NaHCO3: no
sodium pyruvate: no
phenol red: no

shipped in

ambient

storage temp.

2-8°C

General description

Minimum Essential Medium Eagle contains 19 amino acids. The essential amino acids and the non-essential amino acids: L-ala, L-asn, L-asp, L-glu, L-gly, L-pro, and L-ser. This medium is preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium. It is suitable for a wide variety of cells grown in monolayers.

Application

Minimum Essential Medium Eagle has been used:

  • to culture endometrial adenocarcinoma Ishikawa cells for bisphenol studies
  • to culture MCF-7 cells for experimental treatments with hormone
  • to maintain zebrafish pineal glands for action spectrum analysis

Features and Benefits

Contains high concentrations of amino acids so the medium resembles the protein composition of cultured mammalian cells

Preparation Note

Formulated to contain 9.3 grams of powder per liter of medium.
Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.


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저장 등급

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문


문서

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.


Kyle S Enger et al.
Journal of water and health, 14(6), 950-960 (2016-12-14)
Many different household water treatment (HWT) methods have been researched and promoted to mitigate the serious burden of diarrheal disease in developing countries. However, HWT methods using bromine have not been extensively evaluated. Two gravity-fed HWT devices (AquaSure™ and Waterbird™)
Anatoli Y Kabakov et al.
PloS one, 10(4), e0123718-e0123718 (2015-04-24)
During long-term potentiation (LTP) of excitatory synapses, Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is activated by Ca(2+) influx through NMDA receptors that potentiate AMPA receptor currents by insertion of additional GluR1-containing receptors at the synapse and by increasing AMPA channel conductance
Hongjie Fan et al.
Cells, 10(11) (2021-11-28)
Bisphenol A (BPA) is a well-known endocrine disruptor, widely used in various consumer products and ubiquitously found in air, water, food, dust, and sewage leachates. Recently, several countries have restricted the use of BPA and replaced them with bisphenol S