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크기 선택
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C6H13NO4S
CAS 번호:
Molecular Weight:
195.24
NACRES:
NA.25
PubChem Substance ID:
eCl@ss:
32129211
UNSPSC Code:
12161700
EC Number:
224-632-3
MDL number:
Beilstein/REAXYS Number:
141319
SMILES string
OS(=O)(=O)CCN1CCOCC1
InChI
1S/C6H13NO4S/c8-12(9,10)6-3-7-1-4-11-5-2-7/h1-6H2,(H,8,9,10)
InChI key
SXGZJKUKBWWHRA-UHFFFAOYSA-N
assay
≥99% (titration)
form
powder
storage condition
dry at room temperature
impurities
≤1.0% water
color
white
pH
2.5-4 (25 °C, 97.6 g/L)
useful pH range
5.5-6.7
pKa
6.1
density
1.23 g/cm3 at 20—25 °C, 1.27 g/cm3 at 20—25 °C
application(s)
clinical research
diagnostic assay manufacturing
life science and biopharma (
)
metabolomics (
)
Quality Level
General description
MES Buffer, recognized as one of the "Good" buffers employed in biological and biochemical applications, plays a crucial role across various research domains. This zwitterionic and morpholinic buffer proves highly valuable due to its effective buffering capacity within the pH range of 5.5 to 6.7. Notably, MES Buffer does not engage in complex formation with the majority of metals commonly employed in environmental and biological studies. Its solubility in water is excellent, and it exhibits minimal lipid solubility, rendering it impermeable to membranes.
This versatile buffer may find widespread application in maintaining a stable environment in solutions dedicated to cell culture media, protein-based buffer formulations, and electrophoresis running buffers. Its significance extends to the purification processes of antibodies, peptides, proteins, blood components, and growth factors. MES Buffer stands out as an optimal choice for capillary electrochromatography, offering low ionic mobility. Notably, it serves as a safer alternative to potentially toxic agents like cacodylate and non-zwitterionic buffers, including citrate and malate. MES Buffer also proves valuable for fine-tuning the pH of growth media, stabilizing enzymatic solutions, and serving as a component of PAGE running buffer in diverse experimental settings.
This versatile buffer may find widespread application in maintaining a stable environment in solutions dedicated to cell culture media, protein-based buffer formulations, and electrophoresis running buffers. Its significance extends to the purification processes of antibodies, peptides, proteins, blood components, and growth factors. MES Buffer stands out as an optimal choice for capillary electrochromatography, offering low ionic mobility. Notably, it serves as a safer alternative to potentially toxic agents like cacodylate and non-zwitterionic buffers, including citrate and malate. MES Buffer also proves valuable for fine-tuning the pH of growth media, stabilizing enzymatic solutions, and serving as a component of PAGE running buffer in diverse experimental settings.
Application
It has been utilized as a buffer bicarbonate‐containing culture medium
MES has been used as a component of the elution buffer used to elute plasma from α2-macroglobulin affinity column. It has been used as an activation buffer during antibody conjugation to microparticles. MES has been used in the functionalization of microchannels during the creation of microfluidic-integrated surface plasmon resonance (SPR) platform for pathogen detection.
Features and Benefits
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Effective Buffering from pH 5.5-6.7 (25 °C) with a pKa of 6.1 (25 °C)
- Highly soluble in water
- Minimal metal ion binding
- Less toxic to cells than other buffers such as Tris and phosphate
- Stable in a wide pH range
- Low UV absorptivity and Minimal reactivity
Analysis Note
Low moisture
Other Notes
For additional information on our range of Biochemicals, please complete this form.
저장 등급
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
Portable microfluidic integrated plasmonic platform for pathogen detection
Tokel, Onur, et al.
Scientific Reports, 2015, 9152-9152 null
Impaired lipoprotein receptor-mediated peripheral binding of plasma amyloid-? is an early biomarker for mild cognitive impairment preceding Alzheimer's disease.
Sagare, Abhay P., et al.
Journal of Alzheimer'S Disease, 24 (1), 25-34 (2011)
Sensitive Mie scattering immunoagglutination assay of porcine reproductive and respiratory syndrome virus (PRRSV) from lung tissue samples in a microfluidic chip
Song, Jae-Young, et al.
J. Virol. Methods, 178, 31-38 (2011)
"(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review.
Ferreira, Carlos MH, et al.
Royal Society of Chemistry Advances, 5 (39), 30989-31003 (2015)
Sensitive Mie scattering immunoagglutination assay of porcine reproductive and respiratory syndrome virus (PRRSV) from lung tissue samples in a microfluidic chip.
Song JY, Lee CH, Choi EJ, Kim K, et al.
J. Virol. Methods, 178, 31-38 (2011)
프로토콜
폴리아크릴아미드 겔 화학의 개요와 mPAGE® TurboMix Bis-Tris 겔 캐스팅 키트를 이용한 폴리아크릴아미드 겔 핸드캐스팅에 대한 상세 지침
Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.
관련 콘텐츠
MES Specification Comparison
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