M5650
Minimum Essential Medium Eagle
With non-essential amino acids, Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
동의어(들):
EMEM, MEM
제품 이름
Minimum Essential Medium Eagle, With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
shipped in
ambient
storage temp.
2-8°C
Quality Level
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General description
Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. This formulation has been further modified by the optional elimination of calcium to permit the growth of cells in suspension culture. MEM can be used for the cultivation of a wide variety of cells grown in monolayers.
Application
Minimum Essential Medium Eagle has been used to culture:
This medium may be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.
- HeLa cells
- Madin-Darby canine kidney (MDCK) cells
- chondrosarcoma cell line
This medium may be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.
Preparation Note
Supplement with 0.292 g/L L-glutamine.
저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Christopher G Mills et al.
Scientific reports, 7(1), 14824-14824 (2017-11-03)
We present a strategy for increasing the anatomical realism of organoids by applying asymmetric cues to mimic spatial information that is present in natural embryonic development, and demonstrate it using mouse kidney organoids. Existing methods for making kidney organoids in
Junjie Jing et al.
International journal of clinical and experimental medicine, 8(8), 13127-13135 (2015-11-10)
Tectonic family member 1 (TCTN1) encodes a member of the tectonic family which are evolutionarily conserved secreted and transmembrane proteins, involving in a diverse variety of developmental processes. It has been demonstrated that tectonics expressed in regions that participate in
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Oyama T, et al.
Materials & Design, 208, 109975-109975 (2021)
Bryan Tay et al.
PloS one, 14(3), e0213751-e0213751 (2019-03-12)
Voltage-gated sodium channels (NaVs) are key therapeutic targets for pain, epilepsy and cardiac arrhythmias. Here we describe the development of a no-wash fluorescent sodium influx assay suitable for high-throughput screening and characterization of novel drug leads. Addition of red-violet food
Emanuela Profilo et al.
Biochimica et biophysica acta, 1863(6), 1422-1435 (2017-02-27)
The mitochondrial aspartate-glutamate carrier isoform 1 (AGC1) catalyzes a Ca
문서
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
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