M5655
Thiazolyl Blue Tetrazolium Bromide
powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
동의어(들):
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C18H16BrN5S
CAS 번호:
Molecular Weight:
414.32
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
EC Number:
206-069-5
Beilstein/REAXYS Number:
4081397
MDL number:
InChI key
AZKSAVLVSZKNRD-UHFFFAOYSA-M
InChI
1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
SMILES string
[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4
product line
BioReagent
assay
≥97.5% (HPLC)
form
powder
technique(s)
cell culture | insect: suitable, cell culture | mammalian: suitable
mp
195 °C (dec.) (lit.)
solubility
H2O: 5 mg/mL
storage temp.
2-8°C
Quality Level
유사한 제품을 찾으십니까? 방문 제품 비교 안내
Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm.
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
Thiazolyl Blue Tetrazolium Bromide has been used as a colorimetric metabolic activity indicator in cell viability assays (MTT assay). It has also been used to determine cell proliferation.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Fabrication, mechanical properties, and biocompatibility of graphene-reinforced chitosan composites
Fan H, et al.
Biomacromolecules, 11(9), 2345-2351 (2010)
Sahar Yarahmadi et al.
Gene, 711, 143939-143939 (2019-06-21)
Sirtuin 1 is one of the regulators of cell growth and survival and its inhibition is suggested as a suitable mechanism to overcome breast cancer development. In this study we explored the role of miR-211-5p in SIRT1/p53 pathway and its
UNC569, a novel small-molecule mer inhibitor with efficacy against acute lymphoblastic leukemia in vitro and in vivo
Christoph S, et al.
Molecular Cancer Therapeutics (2013)
Nanoparticles made from novel starch derivatives for transdermal drug delivery
Santander Ortega MJ, et al.
Journal of Controlled Release : Official Journal of the Controlled Release Society, 141(1), 85-92 (2010)
Dasatinib synergizes with doxorubicin to block growth, migration, and invasion of breast cancer cells
Pichot CS, et al.
British Journal of Cancer, 101(1), 38-38 (2009)
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.