M6018
4-Methylumbelliferyl β-D-cellobioside
glucanase substrate, fluorogenic, ≥98.00% (TLC), powder
동의어(들):
4-Methylumbelliferyl beta-D-cellobioside
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C22H28O13
CAS 번호:
Molecular Weight:
500.45
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
276-744-7
MDL number:
제품 이름
4-Methylumbelliferyl β-D-cellobioside, glucanase substrate
Quality Level
assay
≥98.00% (TLC)
form
powder
solubility
water: 19.60-20.40 mg/mL, clear, colorless to faintly yellow
storage temp.
2-8°C
SMILES string
CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)ccc12
InChI
1S/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15-,16+,17-,18-,19-,20-,21-,22+/m1/s1
InChI key
PRTGXBPFDYMIJH-MKQZUAMYSA-N
Application
4-Methylumbelliferyl β-D-cellobioside has been used as a substrate:
- to assay cellulase from A. niger
- to determine the extracellular enzyme activity for 1,4-β-cellobiohydrolase
- to assay the activity of bound enzyme, endoglucanase I (EGIc) Trichoderma reesei class II hydrophobins (HFBI)
Biochem/physiol Actions
4-Methylumbelliferyl β-D-cellobioside is used as a substrate for cellulase assays. It is mainly used for activity staining of cellulases in polyacrylamide gels.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
관련 콘텐츠
Product Information Sheet
M Pedraza-Reyes et al.
Archives of microbiology, 168(4), 321-327 (1997-09-23)
The gram-negative bacterium Myxobacter sp. AL-1 produces chitosanase-cellulase activity that is maximally excreted during the stationary phase of growth. Carboxymethylcellulase zymogram analysis revealed that the enzymatic activity was correlated with two bands of 32 and 35 kDa. Ion-exchange-chromatography-enriched preparations of
A V Gusakov et al.
Biochimica et biophysica acta, 1073(3), 481-485 (1991-04-09)
Using 4-methylumbelliferyl (MUF) beta-D-cellobioside as a substrate, the ability of cellobiohydrolase I from Trichoderma longibrachiatum to catalyze transglycosylation has been demonstrated. At substrate concentrations greater than 2 mM, the formation of MUF-tetrasaccharide was detected using HPLC. In the course of
K H Jung et al.
Biochemistry and molecular biology international, 44(2), 283-292 (1998-04-08)
A Clostridium thermocellum xylanase gene, designated xynX, was cloned in Escherichia coli and was categorized a novel gene as a result of the comparison of restriction patterns of the C. thermocellum xylanase genes so far reported. The xynX gene encodes