M7278
Minimum Essential Medium Eagle
HEPES modification, with Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
동의어(들):
EMEM, MEM
제품 이름
Minimum Essential Medium Eagle, HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
>7.2
components
L-glutamine: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
Quality Level
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General description
Minimum Essential Medium Eagle comprises sugar, inorganic salts, water-soluble vitamins and essential amino acids. It is widely used as a media formulation for many cell culture studies.
Application
Minimum Essential Medium Eagle has been used for culturing primordial follicles and ovary tissues, human skin fibroblast cells, human colonic epithelial cell line LS174T and hippocampal tissue slices.
Preparation Note
Supplement with 0.292 g/L L-glutamine.
저장 등급
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Samuel Wilson Failor et al.
Neural development, 13(1), 4-4 (2018-03-27)
Activity in neurons drives afferent competition that is critical for the refinement of nascent neural circuits. In ferrets, when an eye is lost in early development, surviving retinogeniculate afferents from the spared eye spread across the thalamus in a manner
Rodrigo Lerchundi et al.
Frontiers in cellular neuroscience, 14, 80-80 (2020-05-07)
Genetically encoded nanosensors such as the FRET-based adenosine triphosphate (ATP) sensor ATeam enable the measurement of changes in ATP levels inside cells, promoting our understanding of metabolic interactions between astrocytes and neurons. The sensors are usually well characterized in vitro
Shafagh A Waters et al.
The EMBO journal, 36(3), 374-387 (2016-11-12)
RNA sequencing studies have identified hundreds of non-coding RNAs in bacteria, including regulatory small RNA (sRNA). However, our understanding of sRNA function has lagged behind their identification due to a lack of tools for the high-throughput analysis of RNA-RNA interactions
Alfred K Yu et al.
Human molecular genetics, 24(10), 2848-2860 (2015-02-06)
Mitochondrial complex I (NADH dehydrogenase) is a major contributor to neuronal energetics, and mutations in complex I lead to vision loss. Functional, neuroanatomical and transcriptional consequences of complex I deficiency were investigated in retinas of the Ndufs4 knockout mouse. Whole-eye
Is it time to reinvent basic cell culture medium?
Turney J McKee et al.
American journal of physiology. Cell physiology, 312(5), C624-C626 (2017-02-24)
문서
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
관련 콘텐츠
Instructions
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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