M7802

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse

Name: Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse
Brand: SIGMA

~2 mg/mL, clone ERK-PT115, purified immunoglobulin, buffered aqueous solution

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD01633612

주요 문서

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제품 이름

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse, ~2 mg/mL, clone ERK-PT115, purified immunoglobulin, buffered aqueous solution

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ERK-PT115, monoclonal

form

buffered aqueous solution

mol wt

antigen, ERK-1 44 kDa
antigen, ERK-2 42 kDa

species reactivity

rat, human

concentration

~2 mg/mL

technique(s)

capture ELISA: suitable
immunocytochemistry: suitable
microarray: suitable
western blot: 0.5 μg/mL using extract of a rat fibroblast cell line, Rat1, activated with sorbitol

isotype

IgG1

UniProt accession no.

P28482

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... MAPK1(5594), MAPK3(5595)
rat ... Mapk1(116590), Mapk3(50689)

Application

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse has been used in:

immunoblotting
western blotting
immunofluorescence
enzyme linked immunosorbent assay (ELISA)
immunochemistry

Biochem/physiol Actions

The antibody reacts specifically with the monophosphorylated threonine and the active diphosphorylated forms of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated form of the MAP kinase molecule, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated threonine residue within the regulatory site of active MAP kinase (e.g.,Thr¹⁸³ in ERK-2).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Activated/ Monophosphorylated (Phosphothreonine ERK-1&2) (mouse IgG1 isotype) is derived from the ERK-PT115 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Mitogen-activated protein kinase 1 (MAPK1) is mapped to human chromosome 22q11.22 and MAPK3 is present on human chromosome 16p11.2.

Immunogen

synthetic peptide HTGFLpTEYVAT, corresponding to the phosphorylated form of ERK-activation loop.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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저장 등급

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

A leak pathway for luminal protons in endosomes drives oncogenic signalling in glioblastoma

Kondapalli KC, et al.

Nature Communications, 6(5), 6289-6289 (2015)

Transcriptional consequences of 16p11. 2 deletion and duplication in mouse cortex and multiplex autism families

Blumenthal I, et al.

American Journal of Human Genetics, 94(6), 870-883 (2014)

Evaluation of the role of MAPK1 and CREB1 polymorphisms on treatment resistance, response and remission in mood disorder patients

Calati R, et al.

Progress in Neuro-Psychopharmacology & Biological Psychiatry, 44, 271-278 (2013)

A Note of Caution: Gramicidin Affects Signaling Pathways Independently of Its Effects on Plasma Membrane Conductance.

Frances Evans et al.

BioMed research international, 2021, 2641068-2641068 (2021-11-02)

Gramicidin is a thoroughly studied cation ionophore widely used to experimentally manipulate the plasma membrane potential (PMP). In addition, it has been established that the drug, due to its hydrophobic nature, is capable of affecting the organization of membrane lipids.

Leader cell positioning drives wound-directed collective migration in TGFbeta-stimulated epithelial sheets

Chapnick DA and Liu X

Molecular Biology of the Cell, 25(10), 1586-1593 (2014)

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Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse

~2 mg/mL, clone ERK-PT115, purified immunoglobulin, buffered aqueous solution

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

제품 이름

biological source

conjugate

antibody form

antibody product type

clone

form

mol wt

species reactivity

concentration

technique(s)

isotype

UniProt accession no.

shipped in

storage temp.

target post-translational modification

Quality Level

Gene Information

설명

Application

Biochem/physiol Actions

Disclaimer

General description

Immunogen

Physical form

안전성 정보

저장 등급

wgk

flash_point_f

flash_point_c

문서

시험 성적서(COA)

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