M7802
Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse
~2 mg/mL, clone ERK-PT115, purified immunoglobulin, buffered aqueous solution
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
ERK-PT115, monoclonal
Application:
ARR, ELISA (c), ICC, WB
Citations:
16
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ERK-PT115, monoclonal
form
buffered aqueous solution
mol wt
antigen, ERK-1 44 kDa, antigen, ERK-2 42 kDa
species reactivity
rat, human
concentration
~2 mg/mL
technique(s)
capture ELISA: suitable, immunocytochemistry: suitable, microarray: suitable, western blot: 0.5 μg/mL using extract of a rat fibroblast cell line, Rat1, activated with sorbitol
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MAPK1(5594), MAPK3(5595)
rat ... Mapk1(116590), Mapk3(50689)
General description
Activated/ Monophosphorylated (Phosphothreonine ERK-1&2) (mouse IgG1 isotype) is derived from the ERK-PT115 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Mitogen-activated protein kinase 1 (MAPK1) is mapped to human chromosome 22q11.22 and MAPK3 is present on human chromosome 16p11.2.
Immunogen
synthetic peptide HTGFLpTEYVAT, corresponding to the phosphorylated form of ERK-activation loop.
Application
Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse has been used in:
- immunoblotting
- western blotting
- immunofluorescence
- enzyme linked immunosorbent assay (ELISA)
- immunochemistry
Biochem/physiol Actions
The antibody reacts specifically with the monophosphorylated threonine and the active diphosphorylated forms of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated form of the MAP kinase molecule, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated threonine residue within the regulatory site of active MAP kinase (e.g.,Thr183 in ERK-2).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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저장 등급
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Evaluation of the role of MAPK1 and CREB1 polymorphisms on treatment resistance, response and remission in mood disorder patients
Calati R, et al.
Progress in Neuro-Psychopharmacology & Biological Psychiatry, 44, 271-278 (2013)
A leak pathway for luminal protons in endosomes drives oncogenic signalling in glioblastoma
Kondapalli KC, et al.
Nature Communications, 6(5), 6289-6289 (2015)
Transcriptional consequences of 16p11. 2 deletion and duplication in mouse cortex and multiplex autism families
Blumenthal I, et al.
American Journal of Human Genetics, 94(6), 870-883 (2014)
Frances Evans et al.
BioMed research international, 2021, 2641068-2641068 (2021-11-02)
Gramicidin is a thoroughly studied cation ionophore widely used to experimentally manipulate the plasma membrane potential (PMP). In addition, it has been established that the drug, due to its hydrophobic nature, is capable of affecting the organization of membrane lipids.
Leader cell positioning drives wound-directed collective migration in TGFbeta-stimulated epithelial sheets
Chapnick DA and Liu X
Molecular Biology of the Cell, 25(10), 1586-1593 (2014)
관련 콘텐츠
Datasheet
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