M9309
McCoy’s 5A Medium
With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
동의어(들):
5A basal medium
제품 이름
McCoy′s 5A Medium, Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: 2.2 g/L
L-glutamine: 0.21 g/L
phenol red: 0.011 g/L
glucose: 3.0 g/L (Dextro)
shipped in
ambient
storage temp.
2-8°C
Quality Level
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General description
The amino acid requirements for in vitro cultivation of Novikoff hepatoma cells were reported by McCoy and colleagues. These experiments were carried out using Basal Medium 5A, which was later modified to create McCoy′s 5A medium.
Application
McCoy′s 5A Medium has been used:
- to culture HCT-116?cells
- to culture clear cell renal cell carcinoma (ccRCC) cells Caki-1
- to maintain and culture human bone osteosarcoma Saos-2 cell line for in vitro cell studies
Biochem/physiol Actions
McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Aleksandr Ianevski et al.
Cancers, 12(6) (2020-07-08)
The evidence that pan-Bcl-2 or Bcl-xL-specific inhibitors prematurely kill virus-infected or RNA/DNA-transfected cells provides rationale for investigating these apoptotic inducers further. We hypothesized that not only invasive RNA or DNA (biological factors) but also DNA/RNA-damaging chemical or physical factors could
Inhibitory effects of adenovirus mediated tandem expression of RhoA and RhoC shRNAs in HCT116 cells.
Xiang-Ping Liu et al.
Journal of experimental & clinical cancer research : CR, 28, 52-52 (2009-04-21)
RhoA and RhoC are deregulated by over expression in many human tumors, including colorectal cancer. Some reports show that they play a pivotal role in the carcinogenesis, tumor development and infiltration metastasis. In this study, for the first time we
Alison Roth et al.
Nature communications, 9(1), 1837-1837 (2018-05-11)
Malaria liver stages represent an ideal therapeutic target with a bottleneck in parasite load and reduced clinical symptoms; however, current in vitro pre-erythrocytic (PE) models for Plasmodium vivax and P. falciparum lack the efficiency necessary for rapid identification and effective
Qiao Zhang et al.
International journal of oncology, 57(1), 197-212 (2020-04-23)
Glucose‑6‑phosphate dehydrogenase (G6PD) is crucial rate‑limiting enzyme of the pentose phosphate pathway (PPP). G6PD dysregulation has been reported in various types of human cancer, and the role of G6PD in cancer progression was demonstrated in numerous studies. A previous study
Naomi D Willis et al.
PloS one, 3(8), e2988-e2988 (2008-08-21)
A-type lamins are type V intermediate filament proteins encoded by the gene LMNA. Mutations in LMNA give rise to diverse degenerative diseases related to premature ageing. A-type lamins also influence the activity of the Retinoblastoma protein (pRb) and oncogenes such
관련 콘텐츠
Instructions
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