N1038
Anti-Nup98 antibody, Rat monoclonal
clone 2H10, purified from hybridoma cell culture
동의어(들):
Anti-Nucleoporin, 98-kD
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Clone:
2H10, monoclonal
Application:
immunocytochemistry
indirect ELISA
western blot
indirect ELISA
western blot
Species reactivity:
rat, human
Citations:
11
Technique(s):
immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using HeLa nuclear extract
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using HeLa nuclear extract
Uniprot accession no.:
제품 이름
Anti-Nup98 antibody, Rat monoclonal, clone 2H10, purified from hybridoma cell culture
biological source
rat
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2H10, monoclonal
form
buffered aqueous solution
mol wt
antigen ~98 kDa
species reactivity
rat, human
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using HeLa nuclear extract
isotype
IgG2cκ
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NUP98(4928)
rat ... Nup98(81738)
Application
Anti-Nup98 antibody, Rat monoclonal may be used in:
- enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
Biochem/physiol Actions
Nucleoporin-98kDa (Nup98) is involved in the translocation of RNA and proteins across the nucleus. It is also a regulator of chromosomal segregation and gene expression. The protein also binds to other nucleoporins and is associated with acute myelogenous leukemia.
Nup98 functions as a docking protein for cytosol mediated docking of import substrates. Securin protein damage caused by the anaphase-promoting complex (APC) during mitosis, is regulated by the nucleocytoplasmic transport factors Rae1 and Nup98. In mice, collective haploinsufficiency of Rae1 and Nup98 gene causes premature separation of sister chromatids and severe aneuploidy. In addition, it also leads to untimely degradation of securin. Chromosome rearrangement between the HOXA9 (homeobox A9) gene and the nucleoporin gene Nup98 has been observed in some human myeloid leukemia patients.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Nup98 (rat IgG2c- isotype) is derived from the hybridoma 2H10 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from WKY/NCrj rat immunized with a recombinant fragment of human Nup98. Nup96 and Nup98 are the nucleoporins that are formed by the cleavage of 186 kDa precursor protein.
Nucleoporin-98kDa (Nup98) is part of the nuclear pore complex. It possesses two domains and the gene encoding it is localized on human chromosome 11.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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저장 등급
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Céline Ricour et al.
Journal of virology, 83(21), 11223-11232 (2009-08-28)
The leader protein of cardioviruses, Theiler's murine encephalomyelitis virus (TMEV) and encephalomyocarditis virus (EMCV), is a multifunctional protein known to antagonize type I interferon expression and to interfere with nucleocytoplasmic trafficking of host proteins and mRNA. This protein plays an
Masaaki Iwamoto et al.
Genes to cells : devoted to molecular & cellular mechanisms, 15(7), 661-669 (2010-06-16)
The nucleoporin Nup98 is an essential component of the nuclear pore complex. This peripheral nucleoporin with its Gly-Leu-Phe-Gly (GLFG) repeat domain contributes to nuclear-cytoplasmic trafficking, including mRNA export. In addition, accumulating studies indicate that Nup98 plays roles in several important
Takaomi Fukuhara et al.
Hybridoma (2005), 24(5), 244-247 (2005-10-18)
Nup98 is a component of nuclear pore complexes, which are large protein assemblies embedded in the nuclear envelope. Previous studies have shown that Nup98 interacts with several transport factors and plays a critical part in nuclear trafficking. However, the mechanism
NUP98 gene fusions and hematopoietic malignancies: common themes and new biologic insights
Gough SM, et al.
Blood, 118(24), 6247-6257 (2011)
Marcela Vaz et al.
Cells, 10(11) (2021-11-28)
Chromosomal translocations involving the nucleoporin NUP98 gene are recurrently identified in leukemia; yet, the cellular defects accompanying NUP98 fusion proteins are poorly characterized. NUP98 fusions cause changes in nuclear and nuclear envelope (NE) organization, in particular, in the nuclear lamina
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