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Merck

N2640

4-Nitrophenyl phosphate disodium salt hexahydrate

chromogenic, tablet

동의어(들):

p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS 번호:
333338-18-4
Molecular Weight:
371.14
NACRES:
NA.83
PubChem Substance ID:
24897580
UNSPSC Code:
12352204
EC Number:
224-246-5
MDL number:
MFCD00066288
Beilstein/REAXYS Number:
5324661

주요 문서

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제품 이름

4-Nitrophenyl phosphate disodium salt hexahydrate, tablet

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

form

tablet

solubility

water: 1 tablet/3 mL, clear, colorless

storage temp.

−20°C

Quality Level

200

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관련 카테고리

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used in enzyme-linked immunosorbent assay (ELISA) as a substrate for streptavidin-Alkaline Phosphatase. It has also been used as a substrate in control assay for comparing 4-aminophenyl phosphate/diethanolamine (APP/DEA) assay for alkaline phosphatase.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.

Preparation Note

Contains 15 mg substrate per tablet

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
0000467468
0000467468
0000439116
0000439116
0000439125

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문서 라이브러리 방문

Christopher J Sample et al.
Peptides, 48, 96-105 (2013-07-31)
Broad-spectrum antiviral drugs are urgently needed to treat individuals infected with new and re-emerging viruses, or with viruses that have developed resistance to antiviral therapies. Mammalian natural host defense peptides (mNHP) are short, usually cationic, peptides that have direct antimicrobial
Vitamin A differentially regulates cytokine expression in respiratory epithelial and macrophage cell lines
Penkert RR, et al.
Cytokine, 91, 1-5 (2017)
Protein tyrosine phosphatase assays
Lorenz U
Current Protocols in Immunology, 93(1), 11-17 (2011)
Alkaline Phosphatase Assay Based on the Chromogenic Interaction of Diethanolamine with 4-Aminophenol
Sun J, et al.
Analytical Chemistry, 90(10), 6339-6345 (2018)
Christina Papista et al.
Kidney international, 88(2), 276-285 (2015-03-26)
IgA1 complexes containing deglycosylated IgA1, IgG autoantibodies, and a soluble form of the IgA receptor (sCD89), are hallmarks of IgA nephropathy (IgAN). Food antigens, notably gluten, are associated with increased mucosal response and IgAN onset, but their implication in the
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