N3013
Nile Red
Powder
동의어(들):
9-(diethylamino)-5H-benzo[a]phenoxazin-5-one, 9-(diethylamino)benzo[a]phenoxazin-5-one, Nile Blue A Oxazone
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C20H18N2O2
CAS 번호:
Molecular Weight:
318.37
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
230-966-0
MDL number:
Beilstein/REAXYS Number:
279110
제품 이름
Nile Red, Technical grade
InChI
1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
SMILES string
CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34
InChI key
VOFUROIFQGPCGE-UHFFFAOYSA-N
grade
technical grade
form
powder
composition
Carbon: 70.0-77.3%
Nitrogen: 7.5-8.8%
technique(s)
titration: suitable
mp
203-205 °C (lit.)
solubility
methanol: 1 mg/mL
λmax
553 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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General description
Nile Red is a lipophilic fluorescent benzophenoxazone dye that is metachromatic and solvatochromic. It is an uncharged hydrophobic molecule with the ability to stain and visualize lipid droplets in cells and tissues. It functions as a fluorescent probe for intracellular lipids and hydrophobic domains of proteins. This dye is fluorescent in all organic solvents. Its fluorescence colors range from golden yellow to deep red.
Application
Nile red is widely employed in studies related to lipid metabolism, lipid droplet dynamics, lipid accumulation in cells or tissues, and lipid-based drug delivery systems. Its other applications include:
- identification of microplastics in routine analysis of biological samples.
- detection of lysosomes and lysosome-related organelles, like gut granules in C. elegans intestinal cells.
- it exhibits strong fluorescence enhancement and is used for staining SDS (sodium dodecyl sulphate) gels.
Biochem/physiol Actions
Nile Red is lipophilic in nature and exploits the hydrophobic properties of molecules such as lipids and plastics that fluoresce when excited with certain wavelengths, facilitating quantification and identification.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Luca Nalbone et al.
Marine pollution bulletin, 172, 112888-112888 (2021-08-29)
Nile Red is a lipophilic, metachromatic and solvatochromic dye used as an alternative or complementary method to aid identification of microplastics in routine analysis of biological samples. It was rarely used in biota since organic residues after the digestion step
Zebrafish obesogenic test: a tool for screening molecules that target adiposity.
Tingaud-Sequeira A
Journal of Lipid Research, 52, 1765-1765 (2011)
D L Sackett et al.
Analytical biochemistry, 167(2), 228-234 (1987-12-01)
Nile red is an uncharged hydrophobic molecule whose fluorescence is strongly influenced by the polarity of its environment. It interacts with many, but not all, native proteins, including beta-lactoglobulin, kappa-casein, and albumin, with a wide range of spectral changes for
Protein gel staining methods: an introduction and overview
Thomas H Steinberg
Methods in Enzymology, 541-563 (2009)
Hanna Fares et al.
Methods in cell biology, 107, 239-263 (2012-01-10)
This chapter describes methods for studying membrane traffic and organelle biogenesis in Caenorhabditis elegans. These processes have traditionally been studied with yeast or mammalian cells, but C. elegans is emerging as an attractive alternative model system for cell biologists. C.
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