N4645
4-Nitrophenyl phosphate disodium salt hexahydrate
powder, BioReagent, suitable for cell culture, ≥97%
동의어(들):
p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS 번호:
Molecular Weight:
371.14
UNSPSC Code:
12352204
NACRES:
NA.75
PubChem Substance ID:
EC Number:
224-246-5
Beilstein/REAXYS Number:
5324661
MDL number:
InChI key
KAKKHKRHCKCAGH-UHFFFAOYSA-L
InChI
1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
SMILES string
O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
biological source
synthetic (organic)
product line
BioReagent
assay
≥97%
form
powder
technique(s)
cell culture | mammalian: suitable
solubility
H2O: 100 mg/mL
storage temp.
−20°C
Quality Level
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General description
Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
Application
4-Nitrophenyl phosphate disodium salt hexahydrate has been used:
- as a component in diethanolamine buffer to detect alkaline phosphatase-conjugated antibodies in enzyme linked immunosorbent assay (ELISA)
- to treat adherent cells in adhesion assay
- as a phosphatase substrate in in vitro phosphatase assay in substrate buffer for ELISA
Biochem/physiol Actions
p-Nitrophenyl phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in enzyme linked immunosorbent assay (ELISA) procedures. It is the substrate of choice in alkaline phosphatase systems due to its high sensitivity. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. This product is tested for use in cell culture applications. 4-nitrophenyl phosphate (pNPP), a small-molecule substrate can be used to study on alkaline phosphatases, acid phosphatases etc.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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관련 콘텐츠
Product Information Sheet
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