N8630
Nuclease P1 from Penicillium citrinum
lyophilized powder, ≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)
동의어(들):
3′-Phosphohydrolase, Nuclease 5′-Nucleotidehydrolase, Endonuclease P1
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
제품 이름
Nuclease P1 from Penicillium citrinum, lyophilized powder, ≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)
biological source
Penicillium citrinum
form
lyophilized powder
specific activity
≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)
secondary activity
≥1,000 units/mg protein 3′-nucleotidase
mol wt
42-50 kDa
packaging
vial of ≥250 units (using RNA substrate)
technique(s)
DNA extraction: suitable
DNA purification: suitable
suitability
suitable for molecular biology
application(s)
cell analysis
storage temp.
2-8°C
Quality Level
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Application
- Nuclease P1 cleave of single stranded DNA or RNA to 5′ mononucleotides
- Nuclease P1 supports DNA damage and modification research
- Nucleic acids base composition and structural analysis can be done by Nuclease P1
- Nuclease P1 has historically been used for the industrial production of 5′-mononucleotides from yeast RNA.
- Removal of nucleic acids through protein purification can be done by Nuclease P1
- Nuclease P1 is a key reagent for the development of methods for studies involving t-RNA dependent amino acid biosynthesis and t-RNA dependent trans-amidation
- Nuclease P1 from Penicillium citrinum has been used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent.
- Nuclease P1 was used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5′ or 3′ end group labeled RNA.
- Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts.
The enzyme has an optimal temperature of approximately 70 °C, but for a long incubation, a temperature below 60 °C is more suitable. It is stable in the pH range of 5 - 8.
Biochem/physiol Actions
Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phosphooligonucleotides. It does not appreciably degrade double-stranded nucleic acids, especially in the presence of more than 400 mM sodium chloride at pH 6.0.
Features and Benefits
Our highly active Nuclease P1 is tested for its 3′- 5′ - Phosphodiesterase Activity and 3′- Nucleotidase Activity and is the most active Nuclease P1 in the market
General description
A zinc dependent glycoprotein consisting of 270 amino acid residues. Molecular mass: 42-50 kDa.
Nuclease P1 is one of the most commonly known single-strand specific nucleases in molecular biology; Nuclease P1 is a single stranded specific endoduclease (of ssDNA or ssRNA). Nuclease P1 can also cleave single-stranded regions in double-stranded nucleic acids.
Nuclease P1 from Penicillium citrinum is a zinc-dependent endonuclease that exhibits increased activity in the presence of low concentrations of urea. Nuclease P1 selective activity has found useful applications in studies on nucleic acid structure.
Nuclease P1 from Penicillium citrinum is a zinc-dependent endonuclease that exhibits increased activity in the presence of low concentrations of urea. Nuclease P1 selective activity has found useful applications in studies on nucleic acid structure.
Other Notes
3′-5′-Phosphodiesterase: One unit will liberate 1.0 μmole of acid soluble nucleotides from RNA per min at pH 5.3 at 37 °C.
3′-Nucleotidase: One unit will hydrolyze 1.0 μmole of orthophosphate from 3′-AMP per min at pH 7.2 at 37 °C.
3′-Nucleotidase: One unit will hydrolyze 1.0 μmole of orthophosphate from 3′-AMP per min at pH 7.2 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Xiaohuan Jin et al.
Nucleic acids research, 47(2), 883-898 (2018-12-07)
Modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m1G) on position 37; a function mediated by Trm5 homologs. We show that AtTRM5a (At3g56120) is a Trm5 ortholog in Arabidopsis thaliana.
A Lahm et al.
Journal of molecular biology, 215(2), 207-210 (1990-09-20)
P1 nuclease, a zinc-dependent single-strand specific endonuclease from Penicillium citrinum, has been crystallized in three different space groups using either ammonium sulphate or polyethylene glycol 4000 as the precipitating agent. The crystals diffract to between 3 A and 2.2 A.
Fahimeh Salehi et al.
Scientific reports, 8(1), 13902-13902 (2018-09-19)
DNA targeting anticancer agents have been very successful in clinic, especially, when used in combinatorial therapy. But unfortunately, they often exhibit high levels of toxicity towards normal cells. Hence, much effort has been put into finding agents with more selectivity
M V Reddy et al.
Carcinogenesis, 7(9), 1543-1551 (1986-09-01)
Exceedingly sensitive procedures are required to detect the presence of covalent DNA adducts in humans exposed to environmental genotoxicants because of low levels of such derivatives (1 adduct in 10(8)-10(10) DNA nucleotides). A 32P-postlabeling assay for detection and quantitation of
Gerard Mazón et al.
Nature structural & molecular biology, 19(9), 964-971 (2012-08-14)
Holliday junctions can be formed during homology-dependent repair of DNA double-strand breaks, and their resolution is essential for chromosome segregation and generation of crossover products. The Mus81-Mms4 and Yen1 nucleases are required for mitotic crossovers between chromosome homologs in Saccharomyces
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